Physiological significance of TRPV2 as a mechanosensor, thermosensor and lipid sensor
The Journal of Physiological Sciences volume 66, pages 359–365 (2016)
This review provides a summary of the physiological significance of the TRPV2 ion channel. While TRPV2 was initially characterized as a noxious heat sensor, we found that TRPV2 can also act as a mechanosensor in embryonic neurons or adult myenteric neurons. Here, we summarize the newly characterized functions of TRPV2, including the research progress that has been made toward our understanding of TRPV2 physiology, and discuss other recent data pertaining to TRPV2. It is thought that TRPV2 may be an important drug target based on its broad expression patterns and important physiological roles. The possible associations between diseases and TRPV2 are also discussed.
Members of the transient receptor potential (TRP) superfamily are widely expressed throughout numerous tissues. The channels are activated by various stimuli such as temperature, light, chemical and physical stimuli. TRP channels were originally identified in Drosophila photoreceptors , from which the first channel was cloned . The TRP channels are expressed in a range of species from yeast to humans. Most TRP channels act as polymodal receptors. For example, some TRP channels expressed in the nociceptive sensory neurons of the dorsal root ganglion (DRG) generate pain . Such TRP channels represent leading targets for the cure of acute and chronic pain [3–5]. Given this importance, the recent advances in our understanding of TRPV1 and TRPA1 have been impressive; these include the characterization of channel structure [6, 7], identification of ligands, and modulation of channels and intracellular signaling related to pain . Beyond pain sensation, TRP channels have other important functions, or are involved in pathological processes such as diabetes, cancer and other genetic disorders . TRP channels have a common membrane structure with six transmembrane segments (S1–S6) containing a TRP domain and a loop between S5 and S6, which defines the pore and selectivity filter (Fig. 1a) . Similar to other voltage-gated potassium channels, TRP tetramers form the functional non-selective cation channels (Fig. 1b). The N- and C-terminal cytoplasmic domains are important for channel gating . TRP channels are classified by their amino acid sequence identity and similarity, but not by their functional roles. The mammalian TRP channel superfamily is classified into six subfamilies with high sequence similarity (Fig. 1c). The six subfamilies  are TRPV (vanilloid), TRPA (ANKTM1), TRPC (canonical), TRPM (melastatin), TRPML (mucolipin), and TRPP (polycystin). A seventh family, TRPN (NompC) is present in invertebrates and some vertebrates, although this subfamily is absent in mammals . In this review, we focus on the unique characteristics of TRPV2 as a mechanosensor, thermosensor and lipid sensor.
TRPV2 ion channel
The first identified member of the TRPV family was osmotic avoidance abnormal family member 9 (OSM-9) from Caenorhabditis elegans (C. elegans) [9, 11]. This protein was shown to have specific functions for mechanosensation, olfaction and olfactory adaptation. C. elegans has five TRPV channels including OSM-9. In Drosophila, the TRPV channels Nanchung and Inactive are involved in sensory perception . In 1997, TRPV1 was the first TRPV family member identified in mammals using a Ca2+-imaging-based expression cloning method . TRPV1 was shown to be highly expressed in sensory neurons, and pungent compounds such as capsaicin could evoke TRPV1 activation [13, 14]. Based on these studies, it was hypothesized that TRPV1 was related to pain sensation and, indeed, TRPV1KO mice showed impaired pain sensation . TRPV1 is highly expressed in sensory neurons with small diameters which give rise to unmyelinated C-fibers. Given the burning qualities of capsaicin-induced pain, it suggested that capsaicin and heat may evoke painful responses through a common molecular mechanism. As hypothesized, TRPV1 can be activated by heat at >43 °C, a temperature threshold that is consistent with that of heat-evoked pain .
Another molecule with 49 % shared identity to TRPV1 was also isolated and later named TRPV2 [3, 16, 17]. In contrast to TRPV1, TRPV2 is not activated by vanilloids such as capsaicin, protons or thermal stimuli near 43 °C; this is likely attributed to the structure of the ankyrin repeat domains of TRPV2 which are clearly different from those of TRPV1 . However, TRPV2 can be activated by higher temperatures with a threshold of >52 °C. TRPV2 currents showed similar properties to TRPV1 such as an outwardly rectifying I–V relationship and relatively high Ca2+ permeability (P Ca/P Na = 2.9). While translocation of TRPV2 from the cytosol to the plasma membrane by IGF-1 stimulation has been reported , this phenomenon is under debate . In contrast to the expression of TRPV1, TRPV2 is expressed in medium- to large-diameter neurons which give rise to myelinated Aδ- or Aβ-fibers in the DRG . The temperatures at which TRPV2 is activated are more noxious than those that activate TRPV1. This may explain the expression of TRPV2 in myelinated fibers. However, TRPV2KO mice did not display phenotypes which would suggest altered heat sensation . Therefore, further research is required to determine the importance of TRPV2 in the detection of noxious heat stimuli using TRPV2KO mice. TRPV2 expression was not only found in sensory neurons but also in motor neurons and in many non-neuronal tissues [9, 21–23]. These observations indicate that TRPV2 has numerous physiological roles in addition to nociception. For example, we demonstrated that TRPV2 activation regulates the formation of neural circuits in a membrane stretch-dependent manner .
Regulation of axon outgrowth by TRPV2 mechanosensitivity
We have previously demonstrated that TRPV2 expression begins at embryonic day 10 (E10) in mouse DRG sensory and motor neurons . Given that mouse embryos are maintained in a constant temperature environment, we hypothesized that TRPV2 has other functions during the developmental process. Therefore, we examined embryonic stage-specific events which might involve TRPV2, such as cell proliferation, apoptosis, cell migration, and axon outgrowth. While TRPV2 was not found to be related to cell proliferation, apoptosis or cell migration, we did identify a role for TRPV2 in the enhancement of axon outgrowth in developing neurons . We further sought to identify the activators of TRPV2 within developing neurons. Since it was suggested that TRPV2 can be activated by membrane stretch in cardiomyocytes , we hypothesized that membrane stretch resulting from axon outgrowth might endogenously activate TRPV2. To examine this hypothesis, we prepared HEK293T cells expressing mouse TRPV2 and applied artificial membrane stretch (+3 %) to the cells using a pulse-motor-driven stretch machine called STREX  during Ca2+-imaging experiments. The membrane stretch-evoked increase in intracellular Ca2+ ([Ca2+]i) was observed only in transfected (TRPV2-expressing) cells, indicating that TRPV2 can be activated by weak membrane stretch. Consistent with the HEK293T results, most embryonic DRG and motor neurons showed a stretch-evoked [Ca2+]i increase, whereas dominant-negative TRPV2 significantly inhibited this increase . Thus, we demonstrated that developing neurons can sense membrane stretch via TRPV2, and the subsequent activation enhances axon outgrowth (Fig. 2). Moreover, we showed that Ca2+ influx through TRPV2 is necessary for enhancing axon outgrowth. We also demonstrated regulation of axon outgrowth by TRPV2 in vivo using chick embryos . More recently, it has been reported that phosphorylation of TRPV2 by NGF-ERK signaling resulted in the activation of intracellular, but not membranous, TRPV2 as well as enhanced axon outgrowth .
Mechanosensitivity of TRPV2 regulates intestinal motility
We also analyzed the expression of TRPV2 in the mouse intestine . TRPV2-immunoreactive (IR) cells were co-stained with the neuronal marker PGP9.5; however, TRPV2-IR was not observed in GFAP (glial fibrillary acidic protein)-IR enteroglia or KIT-IR ICCs (interstitial cells of Cajal). These results indicated that TRPV2 was expressed in neurons, but not in enteroglia or ICCs. About half of the PGP9.5-IR sensory neurons were TRPV2-positive. Furthermore, most of the nNOS (neuronal nitric oxide synthase)-IR neurons were TRPV2 positive. It has been reported that nNOS-IR neurons are inhibitory circular muscle motor neurons, inhibitory longitudinal muscle motor neurons and descending interneurons. In the inhibitory motor neurons, even slight pressure could evoke TRPV2-mediated current responses, suggesting that enteric neurons expressing TRPV2 might be responsive to weak intestinal distortion. These results strongly suggested that intestinal TRPV2  is also mechanosensitive, similar to its role in the DRG and spinal cord . We examined nitric oxide (NO) production in specimens from the small intestine that were stimulated by a TRPV2 agonist using an enzymatic Griess reaction, and confirmed that TRPV2 activation induces NO release from inhibitory motor neurons which relaxes intestinal tone (Fig. 3) . Furthermore, we also revealed that TRPV2 contributes to transit within the gastrointestinal tract in vivo. Thus, TRPV2 might be a good molecular target for developing a cure for irritable bowel syndrome.
TRPV2 in glial and immune cells
In addition to neurons, glial cells are also important for the maintenance of brain function. Astrocytes provide metabolic support and eliminate waste products, such as neurotransmitters, from the extracellular synaptic space . These glia also regulate blood flow, depending on neuronal activity [28, 29]. Astrocytes are also essential for bidirectional communication with neurons, and thus can modulate neuronal activity [30–33]. We have shown that astrocytes in the brain express functional TRPV2 , and astrocytic TRPV2 is activated by lysophosphatidylcholine (LPC), a known endogenous lipid ligand of TRPV2. In a separate study we demonstrated that another thermo-TRP member, TRPV4, was only expressed in a specific subtype of astrocytes [32, 34]. This astrocytic TRPV4 responded to arachidonic acid, and modulated neuronal excitability through the release of gliotransmitters . During the generation of arachidonic acid at postsynaptic sites, LPC is simultaneously generated, which could affect the excitation of astrocytes through TRPV2 activation. Therefore, astrocytic TRPV2 might be activated in response to increased lipid metabolism at synaptic sites.
Microglial cells also express TRPV2 . Microglia are present throughout the central nervous system (CNS), and are activated by numerous pathogens. The characteristics of microglial cells are very similar to those of macrophages. Using TRPV2KO mice, it was clearly shown that TRPV2 is involved in the phagocytosis of macrophages . It has also been reported that an inflammatory peptide, fMetLeuPhe, could induce membrane-translocation of TRPV2, and TRPV2 is expressed in immune-related tissues such as the spleen [9, 36, 37]. TRPV2 expression is also found in mast cells and lymphocytes [38–40]. One study of mast cells revealed that physical stimuli which lead to cellular degranulation are associated with TRPV2 activation and Ca2+ entry . Thus, these studies highlight the importance of TRPV2 function in numerous immune cell types.
Circulatory function of TRPV2
TRPV2 is also expressed in endothelial cells and the smooth muscle of arteries and veins [41, 42]. It has been reported that hypotonic swelling of aortic myocytes induced Ca2+ influx through TRPV2 activation . Furthermore, it was recently reported that TRPV2-deficient neonatal cardiomyocytes did not form intercalated discs and showed an extracellular Ca2+-dependent increase in intracellular Ca2+ and insulin-like growth factor (IGF-1) secretion in response to stretch stimulation . Moreover, the elimination of TRPV2 from the mouse heart resulted in severe cardiac dysfunction through myocardial conduction defects . Hence, TRPV2 is a critical determinant in the maintenance of cardiac structure and function. Several lines of evidence indicate that TRPV2 acts as a mechanosensor within circulatory organs . However, the mechanisms involved in TRPV2 channel opening by membrane stretch or hypotonic swelling have not been identified. Ankyrin repeats, which are present at the N-terminal region of TRPV2, are thought to be important for mechanosensory function based on the loss of TRPV4 heat-sensitivity by the deletion of its ankyrin repeats . Ankyrin repeats interact with several cytoskeletal proteins ; therefore, the interaction between TRPV2 and the cytoskeleton may be necessary for mechanosensory function. Additionally, it is also possible that membrane stretch or cellular swelling produces an endogenous lipid ligand that activates TRPV2. As described above, LPC is generated during arachidonic acid formation and, when membrane stretch occurs, it induces the generation of arachidonic acid. Therefore, it is highly possible that LPC affects TRPV2 activation .
TRPV2 in the pancreas
TRPV2 is highly expressed in MIN6 cells, a β-type insulinoma cell line. Under unstimulated conditions, TRPV2 is mainly localized within the cytoplasm. In contrast, application of exogenous insulin induces the translocation of TRPV2 from the cytoplasm to the plasma membrane . This translocation evokes Ca2+ entry through TRPV2, resulting in insulin secretion. TRPV2 is also expressed in mouse β-cells prepared from pancreatic islets, but not in α-cells . In addition to insulin, glucose can also induce the translocation of TRPV2 from the cytoplasm to the plasma membrane, and knockdown of TRPV2 reduces insulin secretion. In β-cells, the expression level of TRPV2 in the plasma membrane is also affected by α-Klotho, a known regulator of calcium homeostasis . It was shown that α-Klotho regulates the translocation of TRPV2 from the cytoplasm to the plasma membrane in β-cells, and acts upstream of insulin secretion . These results indicate that pancreatic TRPV2 is an important key regulator of glucose homeostasis.
In this review, we focused on the physiological importance of TRPV2 ion channels as mechanosensors, thermosensors and lipid sensors. TRPV2 is broadly expressed in numerous organ systems (Table 1) where it displays tissue-specific physiological functions. For example, TRPV2 is involved in axon outgrowth, consistent with its cellular localization in the axon shafts and growth cones in embryonic DRG sensory and spinal motor neurons. We have previously demonstrated that TRPV2 has two distinct roles, depending on developmental stage: during embryonic development it regulates axon outgrowth as a mechanosensor  and in adults it regulates thermosensation and/or nociception where it functions as a heat sensor . This was the first characterization of an embryonic stage-specific physiological role for a thermo-TRP channel. We also reported that TRPV2 functions as a mechanosensor in the intestine where it contributes to transit within the gastrointestinal tract . Combined, these results demonstrate that the same channel can have many distinct functions depending on its location. As described above, TRPV2 can also act as a lipid sensor, therefore further identification of novel physiological roles for TRPV2 will be dependent on its pattern of expression. Recently, many reports have indicated that TRPV2 is involved in the disease progression of bladder or prostate cancer [49, 50]. It was found that there was a significant relationship between the overexpression of TRP genes, including TRPV2, and the survival of patients with glioblastoma . These results demonstrate that TRP channels contribute to the progression and survival of glioblastoma patients. Thus, future studies into the physiological function of TRPV2 may reveal new mechanisms behind several diseases.
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The author would like to acknowledge the following funding sources: Grants-in-Aid for Scientific Research (KAKENHI Project No. 15H05934 <Thermal Biology>, 21200012, 20399554, 24111507 + 26111702 <Brain Environment>, 26117502 <glial assembly>, 15H03000) from the Ministry of Education, Culture, Sports, Science and Technology, Japan; a grant from Uehara Memorial Foundation; a grant from Takeda Science Foundation, Tokyo, Japan; a grant from the Sumitomo Foundation; a grant from the Brain Science Foundation; a grant from Narishige Neuroscience Research Foundation; a grant from Salt Science Research Foundation No.14C2; and a grant from the Ichiro Kanehara Foundation.
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Shibasaki, K. Physiological significance of TRPV2 as a mechanosensor, thermosensor and lipid sensor. J Physiol Sci 66, 359–365 (2016). https://doi.org/10.1007/s12576-016-0434-7
- Mechanical stimulus
- Axon outgrowth