Frequency components of systolic blood pressure variability reflect vasomotor and cardiac sympathetic functions in conscious rats

Abstract

In this study, after confirming the suppression of autonomic nervous function by isoflurane anesthesia using autonomic antagonists, we pharmacologically investigated the involvement of vasomotor and cardiac sympathetic functions in systolic blood pressure variability (SBPV) frequency components in conscious rats at rest and during exposure to low-ambient temperature (LT-exposure, 9°C for 90 min). Under unanesthesia, phentolamine administration (α-adrenoceptor antagonist, 10 mg/kg) decreased the mid-frequency component (MF 0.33–0.73 Hz) and inversely increased the high-frequency component (HF 1.3–2.5 Hz). The increased HF was suppressed by subsequent treatment with atenolol (β-adrenoceptor antagonist, 10 mg/kg), but not with atropine (muscarinic receptor antagonist, 10 mg/kg). Moreover, phentolamine administration after atenolol decreased MF, but did not increase HF. LT-exposure increased MF and HF; however, phentolamine pretreatment suppressed the increased MF during LT-exposure, and atenolol pretreatment dose-dependently decreased the increased HF. These results suggest that MF and HF of SBPV may reflect α-adrenoceptor-mediated vasomotor function and β-adrenoceptor-mediated cardiac sympathetic function, respectively, in the conscious state.

Introduction

Blood pressure (BP) and heart rate (HR) continuously fluctuate over time under the influence of control mechanisms such as autonomic nerve activity, humoral factors and respiration for maintaining cardiovascular homeostasis. Among the methods used for analyzing these fluctuations, frequency domain analysis of cardiovascular variability has been used most often as an index of autonomic circulatory control [1]. In particular, heart rate variability (HRV) has become an increasingly popular index for the assessment of autonomic nervous system (ANS) characteristics such as vagal function or cardiac sympatho-vagal balance [2, 3]. However, when evaluating cardiac sympathetic regulation, HRV should be used with discretion since HRV merely reflects the relative value of sympatho-vagal balance; furthermore, the sympathetic and vagal functions are not always reciprocal under all conditions [4]. Systematic and discriminative assessment of vasomotor and cardiac sympathetic functions may be key to understanding complicated pathological conditions because activity of the sympathetic nervous system is regionally specific (heart, vessels, etc.). However, no reliable and accessible tool exists for simultaneous evaluation of vasomotor and cardiac sympathetic nervous function as separate measures.

Information regarding ANS provided by systolic arterial blood pressure variability (SBPV) is different from that provided by HRV. SBPV is commonly analyzed on the basis of low-, mid- and high-frequency components (LF, MF and HF, respectively) of the power spectrum. It has been reported that LF may be mediated by sympathetic and/or humoral factors including the renin-angiotensin system [5], and MF may show strong coherence with synchronous oscillations of post-ganglionic sympathetic nerve activity [6–8] and be decreased by α-adrenoceptor blocker [9–11]. It has been suggested that the genesis of MF may involve in the sympathetic vasomotor component of the arterial baroreceptor reflex; however, it is not known exactly if MF is solely dependent on the α-adrenoceptor-mediated sympathetic nervous system. Experiments under various physical conditions including external stimuli are thus necessary to make MF more applicable. The mechanism underlying HF has not been adequately explained despite several studies indicating that it is a mechanical consequence of respiration [12, 13] and other studies suggesting an association between HF and ANS, particularly the β-adrenergic system [14–16]. To make progress in the availability of SBPV frequency analysis for the evaluation of sympathetic cardiovascular function, the association between the sympathetic nervous system and SBPV must be determined.

Most studies of the involvement of sympathetic cardiovascular function in SBPV (particularly in respiratory HF) are conducted under anesthesia [14–16]; therefore, little is known concerning the natural responses to stimuli under unanesthesia when ANS is not suppressed. It is meaningful to examine the ANS response in a conscious state in order to evaluate complicated pathological conditions (e.g., chronic pain) in human [17] and animal models [18–20].

We hypothesized that MF and HF in SBPV in the conscious state may be involved in the α- and β-adrenergic system, respectively. In the present study, to identify SBPV characteristics, we focused on changes in ANS in a conscious state and explored the link between ANS function and SBPV by means of sequential administration of autonomic antagonists and sympathetic activation stimulus. We first identified differences in autonomic nervous function between anesthetized and unanesthetized states using autonomic antagonists. Then, to elucidate the involvement of vasomotor and cardiac sympathetic functions in SBPV frequency components in conscious rats, we investigated responses to combined administration of autonomic antagonists at rest and in a systemic sympathetic activated state by exposure to low ambient temperature (LT-exposure). A strong relationship between SBPV and α- and β-adrenoceptor-mediated sympathetic function was revealed in conscious rats. Exploration in the frequency domain of cardiovascular regulation in animals could enhance our understanding of the pathology of autonomic dysfunction in humans. Our results suggest that SBPV may be used as a tool for detailed assessment of vasomotor or cardiac sympathetic functions.

Materials and methods

All experiments in this study were carried out according to a protocol approved by the Animal Care Committee at the Aichi Medical University and to the Fundamental Guidelines for Proper Conduct of Animal Experiments and Related Activities in Academic Research Institutions in Japan.

Materials

The experiments were performed using male Sprague-Dawley rats (300–400 g, SLC Inc., Japan). The rats were housed under controlled ambient conditions (23 ± 0.5°C, 50 ± 10%) with a 12-12 h light/dark cycle and provided food and water ad libitum. To minimize effects attributable to circadian alterations, all experiments in this study were conducted between 10 a.m. and 5 p.m. Recordings were performed in a silent room under controlled ambient conditions, as noted above.

Autonomic blockers

Phentolamine mesilate (10 mg/kg), a non-selective α-adrenoceptor antagonist, was obtained from Novartis Pharma (Tokyo, Japan). Atenolol (1 and 10 mg/kg), a selective β1-adrenoceptor antagonist, and atropine methyl bromide (10 mg/kg), a non-selective muscarinic receptor antagonist, were purchased from Sigma (St. Louis, MO). Each drug was dissolved in saline and injected intraperitoneally (i.p.). To examine the effects of drugs on peripheral ANS, we chose these drugs that do not cross the blood-brain barrier. The concentration of each drug (10 mg/kg) was determined in a pilot study: we ensured that each drug provided a sufficient effect over 60 min and gradual recovery 2–3 h after injection.

During this research, we recorded BP continuously for 3 days just after our manipulation (administration of drug or LT-exposure) and examined the effect of the manipulation on the sleep-wake cycle. From the next day after the manipulation, the BP fluctuation pattern returned to normal levels regardless of treatment type. Therefore, we presume that pharmacological manipulation in this study might not have affected the conscious state on the following day of the experiment.

Data acquisition and analysis of cardiovascular parameters

Telemetric monitoring of arterial blood pressure and heart rate

A telemetry system (Primtech Ltd., Japan) was used to continuously record arterial blood pressure (BP) in conscious rats. A radio transmitter equipped with a BP transducer (Physiotel TA11PA-C40, Data Science International, USA) was implanted as follows: (1) under pentobarbital anesthesia (50 mg/kg, i.p.), a catheter connected to the BP transducer was introduced into the abdominal aorta just below the renal artery, (2) the body of the transducer was fixed in the abdominal cavity, and (3) the incision was closed with sutures after antibiotics were sprayed into the abdominal cavity. Continuous recording of BP was initiated at least 1 week after operation in unrestrained and conscious rats.

Electrical signals transformed from BP of rats in each cage were detected by the receiver of the telemetry system, amplified using an electrical circuit in the receiver, and led to a 12-bit A/D converter (PCI-6040E, National Instruments, USA) that digitized the analog signals at a rate of 200 Hz. The converter was controlled by a personal computer. The sampling procedure was programmed using LabVIEW 7.1 (National Instruments). The executable program was developed in our laboratory.

A maximum value corresponding to systolic arterial blood pressure (SBP) and its generation time, which was also utilized to calculate instantaneous heart rate (HR), for each pulse wave of BP were detected, and time series of SBP (TS_SBP) and HR (TS_HR) were constructed. Subsequently, to create a power spectrum with a frequency range up to about 10 Hz by using a continuous wavelet transform, TS_SBP with irregular intervals was reconstructed with a regular interval of 18.2 Hz, so-called resampling.

For this procedure, we used the “Smooth Spline” function in the software R (http://www.r-project.org/), which is a freeware program used for statistics and signal processing.

Frequency analysis of blood pressure fluctuations

With resolutions of time and frequency of about 55 ms and 0.018 Hz, respectively, the power of each frequency at each time point in the variability of TS_SBP was calculated using a continuous Gabor wavelet transform of TS_SBP.

Three prominent peaks of power were frequently observed on this SBPV spectrum in different frequency bands, namely high-frequency (HF 1.3–2.5 Hz), mid-frequency (MF 0.33–0.73 Hz) and low-frequency components (LF 0.04–0.31 Hz). In this study, averaged power values within each component over successive 5-min periods were employed as instantaneous power values for each frequency component at a given time point. On comparing SBPV and the variability of diastolic BP, we found that the power of LF and MF was similar in both variabilities, but that of HF was more prominent in SBPV in our pilot study; this result is consistent with that reported by other studies [21, 22]. Therefore, we used the variability of TS_SBP in this study.

Experimental protocol

Experiment 1: Effects of isoflurane anesthesia on cardiovascular parameters

Data were recorded in 5 rats in the anesthetized and unanesthetized states. In the experiment conducted in the unanesthetized state, a rat was kept singly in an acrylic cage (W 300 mm, H 130 mm, D 205 mm). After recording baseline data for 10 min during the stabilization period, we administered atropine (10 mg/kg, n = 5) or phentolamine (10 mg/kg, n = 5) intraperitoneally. We employed 10-min data for 10–20 min after each injection.

Experiments in the anesthetized state were performed as follows: (1) anesthesia was induced in the rats with 1.3% isoflurane in oxygen and an oral tube was attached, (2) the concentration of anesthesia was changed and maintained at 0.5–0.9% isoflurane in oxygen, (3) after recording 10-min data as baseline (approximately 20 min after administration of 0.5–0.9% isoflurane anesthesia), we administered atropine (10 mg/kg, n = 5) or phentolamine (10 mg/kg, n = 5). The value after the administration was averaged 10-min data for 10–20 min after each administration.

SBPV was analyzed using the 10-min baseline data recorded before administration in the anesthetized (n = 5) and unanesthetized state (n = 5). SBP, HR and each component of SBPV were computed from the BP wave, as described above.

Experiment 2: Effects of autonomic blockers on cardiovascular parameters in conscious rats at rest

A rat was kept singly in the same acrylic cage as that used in Experiment 1. After recording baseline data for 20 min during the stabilization period, we administered phentolamine (10 mg/kg, n = 10) or atenolol (10 mg/kg, n = 10) intraperitoneally as a first injection. A second injection was done 35–40 min after the first injection: atenolol (10 mg/kg, n = 5) or atropine (10 mg/kg, n = 5) after phentolamine treatment; phentolamine (10 mg/kg, n = 5) or atropine (10 mg/kg, n = 5) after atenolol treatment. We then calculated and averaged the 20-min data determined during the 10–30 min period after each injection.

Experiment 3: Effects of autonomic blockers on cardiovascular parameters in conscious rats during exposure to low ambient temperature (LT-exposure)

A rat was kept singly in the same acrylic cage as that used in Experiment 1 placed inside a box made of polystyrene foam (W 350 mm, H 210 mm, D 280 mm). After recording baseline data for 30 min, crushed ice was placed in the space between the cage and the box. The ambient temperature in the cage fell to approximately 9°C from 23°C in about 20 min and was maintained at approximately 9°C for 70 min (total LT-exposure, 90 min). After LT-exposure, the ice was removed, and the temperature returned to the prior level in approximately 25–30 min. In the control group, LT-exposure was performed as described above in rats that were not administered an autonomic blocker (n = 9). In the autonomic blocker groups, the rats were administered phentolamine (10 mg/kg, i.p., n = 5), low-dose atenolol (1 mg/kg, i.p., n = 5) or high-dose atenolol (10 mg/kg, i.p., n = 5) 30 min before LT-exposure. In a pilot study, we confirmed that, when the ambient temperature was changed (20, 15, 9, 7°C), HR showed a temperature-dependent increase and that there was no significant change in HR-increase at 9 and 7°C. Therefore, we presume that the LT-exposure we used (9°C) may be efficient enough to induce high sympathetic activation.

Baseline cardiovascular parameters measured over 30 min before LT-exposure were averaged. We calculated 90-min data recorded throughout LT-exposure because the gradual decrease in temperature induced an ANS response.

Statistical analysis

All data in Experiment 1 are presented as mean ± SEM. SBP and HR in Experiments 2 and 3 are presented as box (median ± interquartile range) and whisker (5 and 95 percentile values) plots, and data values for each SBPV component are expressed as mean ± SEM. A paired t test was employed to compare the data before and after drug administration, data recorded in the anesthetized and unanesthetized states in Experiment 1, before and after administration of the first drug in Experiment 2, and before and during LT-exposure (control) in Experiment 3. Two-way analysis of variance (ANOVA) and Holm’s post hoc tests were utilized to compare treatments with drugs in Experiment 2. To compare all LT-exposure groups in Experiment 3, one-way ANOVA and Holm’s post hoc tests were utilized. Differences were considered statistically significant at the p < 0.05 level.

Results

This experiment revealed pharmacologically that MF and HF components of systolic arterial blood pressure variability (SBPV) changed along with α- and β-adrenoceptor-mediated sympathetic functions, respectively, in conscious rats. Before describing these results, we first report on the effects of isoflurane anesthesia on ANS.

Effects of isoflurane anesthesia on autonomic nervous function

In the anesthetized state, HR and SBP before administration of autonomic blockers were significantly different from those in the unanesthetized state; HR was markedly higher and SBP was significantly lower under anesthesia (Fig. 1a, b; see baseline). Spectral analysis of SBP baseline data showed that LF and MF components of SBPV under anesthesia were significantly lower than those under unanesthesia (LF p = 0.0186, MF p = 0.0191) (Fig. 1c). HF did not differ in the anesthetized and unanesthetized states (Fig. 1c).

Fig. 1

Different effects of the anesthetized (using isoflurane) and unanesthetized states on autonomic nervous function. a Effect of atropine (10 mg/kg, i.p.) on heart rate (HR). b Effects of phentolamine (10 mg/kg, i.p.) on HR and systolic arterial blood pressure (SBP). c Differences in SBP variability (SBPV; 10-min recording data) at baseline (bl). Anesthetized state, n = 5; unanesthetized state, n = 5. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01 and ***p < 0.001 when compared with bl (paired t test); †p < 0.05, ††p < 0.01 and †††p < 0.001 compared with the unanesthetized and anesthetized states (paired t test). PSD power spectrum density, LF low frequency component, MF middle frequency component, HF high frequency component. All abbreviations in subsequent figures are the same as in this figure

Changes in HR as a direct effect of atropine (10 mg/kg, i.p.), which is a parasympathetic blocker to the heart, are shown in Fig. 1a. In the unanesthetized state, atropine administration increased HR (293.7–397.2 bpm, p = 0.0002), as expected. In contrast, in the anesthetized state, HR remained unchanged from the baseline level after atropine administration; thus, it was significantly lower than that in the unanesthetized state. Under anesthesia, HR did not show the direct effect of atropine. Atropine also increased SBP (110.4–123.2 mmHg, p = 0.0159) under unanesthesia, but had only an insignificant effect on SBP under anesthesia (data not shown).

In the unanesthetized state, treatment with phentolamine (10 mg/kg, i.p.) as an α-adrenoceptor-mediated sympathetic blocker significantly decreased SBP (113.9–81.3 mmHg, p = 0.0099) and increased HR (286.3–399.4 bpm, p = 0.0009), which is similar to the response of HR to atropine (Fig. 1a, b). In contrast, in the anesthetized state, phentolamine administration did not induce an increase in HR, despite a significant decrease in SBP (90.0–72.7 mmHg, p = 0.0059) (Fig. 1b). Anesthesia induced changes of baseline in SBP and HR, and also strongly affected HR when the α-adrenoceptor blocker was treated.

Effects of autonomic blockers on cardiovascular parameters in conscious rats at rest

Figure 2 displays typical changes in cardiovascular parameters with subsequent administration of atenolol (10 mg/kg, i.p.) after phentolamine treatment (10 mg/kg, i.p.) in a conscious rat (Experiment 2). Phentolamine treatment decreased SBP and increased HR, as described in the results obtained for the unanesthetized state in Fig. 1b. In terms of frequency components, MF decreased and LF slightly declined, whereas HF was considerably increased by phentolamine administration. These changes were kept stable from 10 min after the administration. Atenolol administration after phentolamine treatment induced sharp and significant decreases in the augmented responses of HR and HF, although other parameters were little affected (Fig. 2).

Fig. 2

Representative data of the time course of changes in cardiovascular parameters following atenolol administration after phentolamine treatment in a conscious rat. Arrows indicate the timing of injections of phentolamine (10 mg/kg, i.p.) and atenolol (10 mg/kg, i.p.)

Averaged data from Experiment 2 are shown in Figs. 3 and 4. Three blockers were administered i.p. in sequential combinations: phentolamine (10 mg/kg) as an α-adrenoceptor antagonist of vascular sympathetic control, atenolol (10 mg/kg) as a β1-adrenoceptor antagonist of cardiac sympathetic control and atropine (10 mg/kg) as a muscarinic antagonist of cardiac parasympathetic control.

Fig. 3

Effects of subsequent administration of atenolol or atropine after phentolamine treatment on cardiovascular parameters in conscious rats: baseline: before treatments (n = 10); phentolamine: after treatment with phentolamine (10 mg/kg, i.p., n = 10) alone; atenolol after phentolamine: after atenolol administration (10 mg/kg, i.p., n = 5) following phentolamine treatment; atropine after phentolamine: after atropine administration (10 mg/kg, i.p., n = 5) following phentolamine treatment. HR and SBP data are presented as box (median ± interquartile range) and whisker (5 and 95 percentile values) plots, and SBPV data are presented as mean ± SEM. Averaged 20-min data were used as baseline data; data of treatment with autonomic blockers were averaged from 20-min data recorded during the 10–30 min after each injection. *p < 0.05, **p < 0.01 and ***p < 0.001 compared with baseline (paired t test); †p < 0.05, ††p < 0.01 and †††p < 0.001 when compared with first and second treatment [two-way analysis of variance (ANOVA) and Holm’s post hoc test]

Fig. 4

Effects of subsequent administration of phentolamine or atropine after atenolol treatment on cardiovascular parameters in conscious rats: baseline: before treatments (n = 10); atenolol: after treatment with atenolol (10 mg/kg, i.p., n = 10) alone; phentolamine after atenolol: after phentolamine administration (10 mg/kg, i.p., n = 5) following atenolol treatment; atropine after atenolol: after atropine administration (10 mg/kg, i.p., n = 5) following atenolol treatment. HR, SBP, and SBPV data are presented as in Fig. 3. In this figure, 20-min data were employed in the same manner as those used in Fig. 3. *p < 0.05, **p < 0.01 and ***p < 0.001 compared with baseline (paired t test);†p < 0.05, ††p < 0.01 and †††p < 0.001 when compared with first and second treatment [two-way analysis of variance (ANOVA) and Holm’s post hoc test]

Figure 3 shows averaged data with responses of cardiovascular parameters following phentolamine pretreatment in conscious rats. Administration of phentolamine alone significantly decreased SBP (108.1–79.6 mmHg, p = 0.0001) and increased HR (282.8–429.6 bpm, p < 0.0001) (Fig. 3); these results are consistent with those observed under unanesthesia in Experiment 1 (Fig. 1b). In terms of frequency components, phentolamine significantly reduced LF (p = 0.0179) and MF (p = 0.0023), and inversely augmented HF (p < 0.0001) (Fig. 3). The increases in HR and HF were markedly suppressed by subsequent treatment with atenolol (HR 454.7–288.8 bpm, p < 0.0001, HF 2.508–0.193 mmHg²/Hz, p < 0.0001) (Figs. 2, 3), but not with atropine (Fig. 3). Atropine administration after phentolamine treatment had only an insignificant effect on all parameters.

Effects of preceding treatment with atenolol on cardiovascular parameters in conscious rats are indicated in Fig. 4. Treatment with atenolol alone led to a decrease in HR (288.5–264.8 bpm, p = 0.0181), but did not affect SBP. In terms of frequency components, atenolol induced a small but significant decrease in HF (p = 0.0175), though no change was observed in LF and MF (Fig. 4). Phentolamine administration after atenolol treatment decreased SBP (111.4–83.3 mmHg, p = 0.0008) in a manner similar to that observed when phentolamine was administered alone (Figs. 1b, 2, 3); however, it did not change HR. As for the frequency components, phentolamine administration after atenolol treatment decreased MF (p = 0.026) in the same way as that observed when phentolamine was administered alone (Figs. 1b, 2, 3), but, unlike phentolamine alone, it did not increase HF (Fig. 4). Augmentation of HF after phentolamine administration occurred only when phentolamine was administered alone, but not when the rats were pretreated with atenolol (Figs. 3, 4), i.e., HF was enhanced only when β-adrenoceptor was intact. In addition, MF decreased only when phentolamine was injected, regardless of pretreatment with other blockers (Figs. 3, 4). Atropine administration after atenolol treatment increased HR (260.8–329.5 bpm, p = 0.0029), but the other parameters remained unchanged (Fig. 4). Throughout this experiment, atropine had little effect on each SBPV component, even after preceding treatment with phentolamine or atenolol (Figs. 3, 4).

Effects of autonomic blockers on cardiovascular parameters during exposure to low ambient temperature (LT-exposure)

The results from Experiment 2 indicated that the changes in MF and HF may be interrelated with administration of phentolamine or atenolol, respectively. In Experiment 3, we examined the responses of cardiovascular parameters to systemic sympathetic activation by exposure to low ambient temperature (LT-exposure) for 90 min (Fig. 5). LT-exposure induced significant increases in SBP (98.6–123.8 mmHg, p < 0.0001) and HR (297.6–446.7 bpm, p < 0.0001). All frequency components of SBPV were also enhanced (LF p = 0.0023, MF p < 0.0001, HF p < 0.0001) by LT-exposure (Fig. 5; see control).

Fig. 5

Effects of autonomic blockers on cardiovascular parameters during exposure to low ambient temperature (LT-exposure): baseline: before LT-exposure (n = 9); control: during LT-exposure without an autonomic blocker (n = 9); atenolol: during LT-exposure after intraperitoneal (i.p.) injection of atenolol (1 mg/kg, n = 5 or 10 mg/kg, n = 5); phentolamine: during LT-exposure after i.p. injection of phentolamine (10 mg/kg, n = 5). HR, SBP and SBPV data are presented as in Fig. 3. Averaged 30-min data were used as baseline data, and 90-min data throughout the exposure period were used for the LT-exposure groups. *p < 0.05, **p < 0.01 and ***p < 0.001 compared with baseline and control (paired t test); †p < 0.05, ††p < 0.01 and †††p < 0.001 compared among LT-exposure groups [one-way analysis of variance (ANOVA) and Holm’s post hoc test]

Effects of the autonomic antagonists on LT-exposure were examined. Augmentation of SBP, LF and MF during LT-exposure was significantly suppressed by phentolamine administration (10 mg/kg, i.p.) before exposure (SBP 123.8–75.7 mmHg, p < 0.0001, LF p = 0.0002, MF p = 0.0001). In contrast, the increase in HR was not reduced in the phentolamine treatment group, and HF was more enhanced than that in the control group (p = 0.0011) (Fig. 5; see control vs. phentolamine).

Atenolol pretreatment significantly suppressed augmentation of HR and HF during LT-exposure, and the effects were dose dependent [HR 371.3 bpm (atenolol 1 mg/kg, i.p.), 325.1 bpm (atenolol 10 mg/kg, i.p.), p < 0.0001 in control vs. 1 mg/kg, p = 0.009 in 1 mg/kg vs. 10 mg/kg; HF: 0.360 mmHg²/Hz (1 mg/kg), 0.175 mmHg²/Hz (10 mg/kg), p = 0.0125 in control vs. 1 mg/kg, p = 0.012 in 1 vs. 10 mg/kg]. Other parameters, SBP, LF and MF in the atenolol pretreatment group were not significantly different from those in the control group (Fig. 5).

In summary, MF was attenuated after phentolamine administration (α-adrenoceptor blocker), and HF was reduced after atenolol administration (β-adrenoceptor blocker).

Discussion

In this study, we investigated HR and SBP responses to three kinds of autonomic blockers under resting and systemic sympathetic activated conditions. Involvement of sympathetic cardiovascular function in SBPV frequency components were explored pharmacologically through the effects of these blockers. We found that each SBPV component has a specific correspondence with vasomotor or cardiac sympathetic function, respectively, in conscious rats.

Suppression of the autonomic nervous system by isoflurane anesthesia

Many studies regarding the involvement of ANS in SBPV have been conducted in the anesthetized state [14–16] because of the simple methods, but it may be important to examine natural responses in the conscious state, in particular, considering sequential measurements of changes in complicated pathological conditions such as chronic pain [18–20].

Isoflurane anesthesia is widely clinically used as an inhalant anesthetic because it is well tolerated. In this study, isoflurane anesthesia induced significant changes of baseline in HR and SBP; HR became higher and SBP became lower than under unanesthesia (Fig. 1a, b). In terms of frequency components of baseline data, the power of LF and MF components of SBPV in the anesthetized state significantly decreased compared to those in the unanesthetized state (Fig. 1c), thus supporting results of other studies [23, 24]. Lower frequency components such as LF and MF are generally considered to be attributed to the influence of sympathetic nervous function [22, 25]; therefore, our results suggest that isoflurane anesthesia may depress ANS function.

Pharmacological experiments conducted under isoflurane anesthesia indicated that atropine acting as a muscarinic receptor antagonist had no effect on HR (Fig. 1a). Given the result that HR in baseline under anesthesia was higher (Fig. 1a, b), isoflurane anesthesia may induce vagal suppression, confirming the results of other studies that reported that respiratory sinus arrhythmia [26] and HR [26, 27] remained unchanged after surgical vagotomy in isoflurane-anesthetized rats. In addition, phentolamine administration (α-adrenoceptor antagonist) in this study induced a decrease in SBP regardless of the induction of anesthesia, but had no effect on HR in the anesthetized state (Fig. 1b). These results suggest that the central nervous system involved in baroreflex may also be depressed by isoflurane anesthesia.

Relationship between MF component and α-adrenoceptor-mediated vasomotor function

At rest, MF in this study was reduced when phentolamine was administered as an α-adrenoceptor blocker (Figs. 2, 3, 4, 5), which is consistent with the results of other studies [9–12]. Phentolamine-induced decreases in MF occurred reliably, regardless of whether phentolamine was administered alone or in combination; MF was consistently unaffected by atenolol administration (β-adrenoceptor antagonist). The MF component of SBPV in this study may have changed solely depending on vasomotor function via the α-adrenoceptor, but not on cardiac sympathetic function. In our preliminary experiment, we confirmed that saline administration induced no significant changes in BP, HR and each component of SBPV (data not shown). Therefore, we did not use saline administration in a control group in this study.

Exposure to low ambient temperature (LT-exposure) in our experiment induced significant augmentation of SBP and HR, which is indicative of systemic sympathetic activation and also confirms the results of other studies [28]. MF of SBPV significantly increased during LT-exposure; however, phentolamine pretreatment suppressed augmentation of SBP and MF (Fig. 5). These results in a systemic sympathetic activated state also indicated a strong relationship between MF and vasomotor function.

The MF component of blood pressure variability (BPV) has been considered to be involved in vasomotor nerve activity via an arterial baroreflex in previous studies of humans [25] and animals [8, 10, 29]. However, the detailed characteristics of MF have remained unclear because the method of sequential administration of different types of autonomic antagonists at rest and in a sympathetic activated state has never been used. Our study revealed the relationship between MF and α-adrenoceptor-mediated vasomotor function using this more comprehensive method.

Relationship between HF component and β-adrenoceptor-mediated cardiac sympathetic function

In this study, the α-adrenoceptor blocker, which induced a decrease in SBP, profoundly enhanced the HF component of SBPV (Figs. 2, 3). A similar effect was observed by other authors [9, 11, 12] and has been considered to be attributed to the mechanical effect of respiration on intrathoracic pressure as blood vessels relax or dilate. Our results, however, showed that the enhanced-HF caused by the α-adrenoceptor blocker was markedly suppressed by subsequent treatment with the β-adrenoceptor blocker (Fig. 3). In addition, results with the β-adrenoceptor blocker indicated that HF did not increase, even when SBP was decreased by the α-adrenoceptor blocker (Fig. 4), i.e., once the β-adrenoceptor was blocked, either before or after the α-adrenoceptor blocker was used, HF remained at a low level. These findings indicate that the HF component may be linked to β-adrenoceptor-mediated cardiac sympathetic function rather than the mechanical effects of respiration.

HF of SBPV is in the same frequency as that of heart rate variability (HRV), which is considered to correspond to respiratory fluctuations. The mechanism underlying the HF respiratory component in the BP wave causes controversy as to whether mechanical effects of respiration [9, 12, 13] or involvement of ANS, particularly in the cardiac sympathetic nervous system [14–16], is involved; however, previous studies that indicated an association between cardiac sympathetic function and HF were performed in the anesthetized state. In our study using conscious animals, we found that HF component appears to be involved in β-adrenoceptor-mediated cardiac sympathetic function rather than respiration.

An increase in the HF component of SBPV in mechanically ventilated, anesthetized rats has been reported to be induced blood-loss-dependently by hemorrhage [11, 16], and the HF component is considered to be an index of blood volume status. Meanwhile, during gradation of hemorrhage or alleviation of myocardial preload, compensation via the sympathetic nervous system generally works as a circulation system. In the light of our results, augmentation of HF after α-adrenoceptor blockade may reflect baroreceptor responses as compensatory activation of the β-adrenoceptor-mediated sympathetic nervous system caused by alleviation of the myocardial preload in the same manner as that observed during hemorrhage.

Atenolol administration alone in this study induced a small but significant decrease in HF (Fig. 4), and atropine administration after atenolol treatment had no effect on HF (Fig. 4). In addition, the amplifying effect of phentolamine on HF was reduced by subsequent treatment with atenolol but not with atropine (Fig. 3). It has been reported that respiratory-related arterial BPV in anesthetized rats is attenuated by the β-adrenoceptor blocker [14–16, 21], but not by vagotomy [14] or atropine [16]. Our results imply that there may be a negative involvement of the vagal function in the genesis of the HF component of SBPV.

We found that the augmented HF induced by LT-exposure was dose-dependently suppressed by β-adrenergic blocker before exposure (Fig. 5). The HF component was enhanced under alleviation of myocardial preload by the α-adrenoceptor blockade and also under augmentation of the preload by LT-exposure (systemic sympathetic activation), i.e., when the cardiac sympathetic nervous system was activated. The β-adrenergic system was indicated to be involved in the genesis of HF in this study. It may be possible that HF of SBPV is applied not only as a specific reflection of a baroreflex compensatory response, but also as a general marker of β-adrenergic sympathetic excitation. In this study, we activated the cardiac sympathetic nervous system indirectly (with use of phentolamine or LT-exposure). In order to establish the method to evaluate various pathological states, it may be necessary to perform further experiments under several conditions, such as using an autonomic agonist or specific animal model in which the β-adrenergic system is enhanced.

Conclusion

In this study, we showed that the MF and HF components of SBPV in conscious rats may reflect the activity of vasomotor and cardiac sympathetic nervous systems, respectively, during systemic sympathetic activation as well as during sympathetic deactivation by autonomic antagonists. Our results also demonstrated that isoflurane anesthesia depressed ANS. We suggest that the MF and HF components of SBPV may act as alternative means of monitoring vasomotor and cardiac sympathetic regulation in a conscious state. Further research is required concerning the link between nerve activity and each component in the unanesthetized states or changes in autonomic nervous function under complicated pathological conditions. Hopefully, such approaches will help enhance the functionality of current BP monitoring and improve our understanding of autonomic dysfunction.