Fig. 5

Epac1 deficiency suppressed intimal thickening and GSK3β phosphorylation. Representative images of immunohistochemistry for GSK3β phosphorylation in the cross-sections of the uninjured (upper panels) and wire-injured (lower panels) femoral arteries of Epac1^+/+ and Epac1^−/− mice. Immunoreaction against GSK3β phosphorylation is shown in green. The nucleus was visualized in blue by Hoechst stain. The area between the white dotted lines indicates intimal thickening. Scale bars, 50 μm