Fig. 3

Epac1 deficiency decreased bFGF-induced phosphorylation of Akt and GSK3β. VSMCs were incubated with or without 10 ng/ml of bFGF for 30 or 60 min. A Representative western blotting images of the phosphorylation of Akt (p-Akt) are shown. B Quantification of A, n = 6. C Representative western blotting images of the phosphorylation of GSK3β (p-GSK3β) are shown. D Quantification of C, n = 6. *p < 0.05; **p < 0.01; ***p < 0.001