ATP-dependent potassium channels and mitochondrial permeability transition pores play roles in the cardioprotection of theaflavin in young rat

Abstract

Previous studies have confirmed that tea polyphenols possess a broad spectrum of biological functions such as anti-oxidative, anti-bacterial, anti-tumor, anti-inflammatory, anti-viral and cardiovascular protection activities, as well as anti-cerebral ischemia-reperfusion injury properties. But the effect of tea polyphenols on ischemia/reperfusion heart has not been well elucidated. The aim of this study was to investigate the protective effect of theaflavin (TF1) and its underlying mechanism. Young male Sprague-Dawley (SD) rats were randomly divided into five groups: (1) the control group; (2) TF1 group; (3) glibenclamide + TF1 group; (4) 5-hydroxydecanoate (5-HD) + TF1 group; and (5) atractyloside + TF1 group. The Langendorff technique was used to record cardiac function in isolated rat heart before and after 30 min of global ischemia followed by 60 min of reperfusion. The parameters of cardiac function, including left ventricular developing pressure (LVDP), left ventricular end-diastolic pressure (LVEDP), maximal differentials of LVDP (±LVdP/dt _max) and coronary flow (CF), were measured. The results showed: (1) compared with the control group, TF1 (10, 20, 40 μmol/l) displayed a better recovery of cardiac function after ischemia/reperfusion in a concentration-dependent manner. At 60 min of reperfusion, LVDP, ±LVdP/dt _max and CF in the TF1 group were much higher than those in the control group, whereas left ventricular end-diastolic pressure (LVEDP) in the TF1 group was lower than that in the control group (P < 0.01). (2) Pretreatment with glibenclamide (10 μmol/l), a K_ATP antagonist, completely abolished the cardioprotective effects of TF1 (20 μmol/l). Also, most of the effects of TF1 (20 μmol/l) on cardiac function after 60 min of reperfusion were reversed by 5-HD (100 μmol/l), a selective mitochondria K_ATP antagonist. (3) Atractyloside (20 μmol/l), a mitochondrial permeability transition pore (mPTP) opener, administered at the beginning of 15 min of reperfusion completely abolished the cardioprotection of TF1 (20 μmol/l). The results indicate that TF1 protects the rat heart against ischemia/reperfusion injury through the opening of K_ATP channels, particularly on the mitochondrial membrane, and inhibits mPTP opening.

Introduction

Several brief repeated ischemia/reperfusion (I/R) cycles before long-term ischemia improve cardiac recovery from I/R injury, which is called ischemic preconditioning (IPC) [1]. The heart's tolerance of ischemia can also be enhanced by some other manipulations, including pharmacological preconditioning [2], cardioplegic protection [3] and hypoxic adaptation [4].

Theaflavins are natural polyphenols found in black tea, including theaflavin (TF1), theaflavin 3-gallate (TF2A), theaflavin 3′-gallate (TF2B) and theaflavin 3,3′-gallate (TF3) [5]. These tea polyphenols possess a broad spectrum of biological functions, such as anti-oxidative, anti-bacterial, anti-tumour, anti-inflammatory, anti-viral and cardiovascular protection activities [6–8]. TF1 has been reported to significantly protect neurons from cerebral I/R injury [9, 10]. The effect of TF1, however, on I/R hearts and the underlying mechanisms are far from clear.

It is well accepted that ATP-dependent potassium (K_ATP) channels activated by ischemic or hypoxic preconditioning protect the heart against I/R injury [11]. It has also been reported recently that inhibition of mitochondrial permeability transition pore (mPTP) opening by ischemia preconditioning (IPC) appears to be associated with cardioprotective effects [12]. So it is reasonable to hypothesize that K_ATP and mPTP may participate in the protective effects of TF1 against I/R injury.

This study was undertaken to evaluate the cardioprotection of TF1, a major constituent of theaflavins, in I/R heart of rats and to investigate the role of K_ATP and mPTP in the cardioprotection of TF1.

Materials and methods

Experiment animal and drugs

Young male Sprague-Dawley (SD) rats weighing 90–120 g were provided by the Experimental Animal Center of Hebei Province, China. All animal experiments were conducted in compliance with the Guide for the Care and Use of Laboratory Animals (National Research Council, 1996). TF1 was purchased from Chromadex Inc., and atractyloside, glibenclamide and 5-hydroxydecanoate (5-HD) were purchased from Sigma (St Louis, MO).

Ischemia/reperfusion in isolated heart

Rats were anesthetized with sodium pentobarbital (50 mg/kg, ip), and the hearts were quickly excised and mounted on a Langendorff apparatus via the aorta for retrograde perfusion with Krebs-Henseleit (K-H) solution at constant pressure (10 kPa). The K-H solution (in mmol/l) was composed of: NaCl 118.0, KCl 4.7, CaCl₂ 2.5, MgSO₄ 1.2, NaHCO₃ 25.0, KH₂PO₄ 1.2 and glucose 11.0. The solution was continuously gassed with 95% O₂ and 5% CO₂ (pH 7.4), and maintained at 37°C. A water-filled latex balloon connected to a pressure transducer (Gould P23Db) was introduced into the left ventricle through the atria to record isovolumic left ventricular pressure. The balloon volume was adjusted to achieve a stable left ventricular end-diastolic pressure (LVEDP) of 3–10 mmHg during initial equilibration. Left ventricular developed pressure (LVDP), LVEDP, the maximal differentials of LVDP (±LVdp/dt _max), heart rate (HR) and coronary flow (CF) were monitored with the PowerLab system (ADInstruments Ltd., Australia), which was similar to that previously described by Zhang et al. [13].

Animal group and experimental protocols

Rats were randomly divided into five groups: (1) control group: after stabilization for 20 min with K-H solution, the hearts were subjected to 30 min no-flow global ischemia followed by 60 min of reperfusion; (2) TF1 group: the hearts were treated with 10, 20 or 40 μmol/l TF1 for 10 min before ischemia and reperfusion, respectively; (3) glibenclamide + TF1 group: the hearts were first perfused for 5 min with 10 μmol/l glibenclamide, a K_ATP antagonist and then treated with 20 μmol/l TF1 and 10 μmol/l glibenclamide together for 10 min before ischemia and reperfusion; (4) 5-hydroxydecanoate (5-HD) + TF1 group: the hearts were first perfused for 5 min with 100 μmol/l 5-HD, a selective mitochondria K_ATP antagonist, and then treated with 20 μmol/l TF1 and 100 μmol/l 5-HD together for 10 min before ischemia and reperfusion; (5) atractyloside + TF1 group: the hearts were treated with 20 μmol/l TF1 for 10 min before ischemia, and atractyloside (20 μmol/l), a mitochondrial permeability transition pore (mPTP) opener, was added at the beginning of 15 min of reperfusion.

Statistical analysis

All data were expressed as mean ± SD. The paired t test was used to compare the data within groups, and ANOVA followed by a Dunnett’s post hoc test was used for data between groups. P < 0.05 was considered significant.

Results

Protective effects of TF1 on I/R rat hearts

There were no significant differences of functional parameters between the control and TF1 groups under non-ischemic conditions. The values of LVDP, +LVdP/dt _max, −LVdP/dt _max and CF decreased, while LVEDP increased significantly in both groups during I/R, which indicates damage of left ventricular function (n = 6, P < 0.05, or P < 0.01, Figs. 1, 2). After 60 min reperfusion, LVDP in TF1 at 10, 20 and 40 μmol/l was 21.8 ± 7.5, 29.4 ± 9.1 and 37.1 ± 9.8 mmHg, respectively, and significantly higher than 18.4 ± 6.7 in the control group; LVEDP was 72.4 ± 6.3, 69.8 ± 6.2 and 58.3 ± 5.6 mmHg, respectively, and significantly lower than 81.8 ± 8.9 in the control group; +LVdP/dt _max was 916.4 ± 176.8, 1,115.4 ± 218.2 and 1,306.2 ± 276.9 mmHg/s, respectively, and significantly higher than 315.5 ± 150.1 mmHg/s in the control group; −LVdP/dt _max was −616.5 ± 106.3, −782.0 ± 164.1 and −1,176.4 ± 134.5 mmHg/s, respectively, and significantly higher than −336.7 ± 171.3 mmHg/s in the control group; CF was 3.3 ± 0.8, 4.5 ± 0.9 and 4.9 ± 1.1 ml, respectively, and significantly higher than 2.8 ± 0.4 ml in the control group (n = 6, Fig. 3, P < 0.05, or P < 0.01). All the above results suggest that TF1 increases the tolerance of hearts against I/R injury in a concentration-dependent manner.

Fig. 1

Original recordings of left ventricular function in isolated rat hearts submitted to 30 min ischemia and 60 min reperfusion. a Control group, b theaflavin (20 μmol/l) group

Fig. 2

The effects of theaflavin (20 μmol/l) on cardiac functional parameters in isolated rat hearts subjected to 30 min of ischemia and 60 min of reperfusion (n = 6 in each group). a LVDP, b LVEDP, c ±LVdP/dt _max. *P < 0.05, **P < 0.01 versus control group

Fig. 3

Effects of theaflavin on cardiac function after I/R (n = 6 in each group), CON control group, a theaflavin (10 μmol/l) group, b theaflavin (20 μmol/l) group, c theaflavin (40 μmol/l) group. *P < 0.05, **P < 0.01 versus control group; ^# P < 0.05, ^## P < 0.01 versus theaflavin (10 μmol/l) group; ^Δ P < 0.05 versus theaflavin (20 μmol/l) group

Influence of glibenclamide and 5-HD on the protective effects of TF1 against I/R injury in isolated rat hearts

After 60 min reperfusion, the LVDP, LVEDP, +LVdP/dt _max, −LVdP/dt _max and CF in the glibenclamide + TF1 group was 17.3 ± 5.1 mmHg, 83.6 ± 10.6 mmHg, 304.2 ± 76.2 mmHg/s, −316.5 ± 21.0 mmHg/s and 3.0 ± 0.6 ml, respectively, and significantly different from 29.4 ± 9.1, 69.8 ± 3.2, 1,115.4 ± 218.2, −562.464.1 and 4.5 ± 0.9 in the TF1 (20 μmol/l) group (n = 6, Fig. 4 P < 0.05, or P < 0.01), but not different from 18.4 ± 6.7 mmHg, 81.8 ± 8.9 mmHg, 315.5 ± 150.1 mmHg/s, −331.1 ± 21.3 mmHg/s and 2.8 ± 0.4 ml in the control group. However, the LVDP, LVEDP, +LVdP/dt _max, −LVdP/dt _max and CF in the 5-HD + TF1 group was 20.7 ± 4.3 mmHg, 78.7 ± 7.7 mmHg, 785.6 ± 163.6 mmHg/s, −411.7 ± 81.8 mmHg/s and 3.6 ± 0.6 ml, respectively, and significantly different from those in the TF1 (20 μmol/l) group (n = 6, Fig. 4, P < 0.05, or P < 0.01), but not different from those in the control group. These data suggest that the cardioprotective effects of TF1 (20 μmol/l) can be abolished by glibenclamide (10 μmol/l) completely, and by 5-HD (100 μmol/l) mostly.

Fig. 4

Influence of K_ATP antagonist and mPTP opener on the protective effects of theaflavin against myocardial ischemia/reperfusion injury (measured after 60 min of reperfusion, n = 6 in each group). CON control group, TF1 theaflavin group (20 μmol/l), GLI glibenclamide group, 5-HD 5-hydroxydecanoate group, ATRA atractyloside group. a LVDP, b LVEDP, c +LVdp/dt _max, d −LVdp/dt _max, e CF. *P < 0.05, **P < 0.01 versus CON group; ^# P < 0.05, ^## P < 0.01 versus TF1 group; ⁺ P<0.05 versus GLI group

Influence of atractyloside on the protective effects of TF1 against I/R injury in isolated rat hearts

After 60 min reperfusion, the LVDP, LVEDP, +LVdP/dt _max, −LVdP/dt _max and CF in the atractyloside + TF1 group were 19.5 ± 5.2 mmHg, 80.4 ± 8.7 mmHg, 318.1673 ± 73.3 mmHg/s, −318.2 ± 22.1 mmHg/s and 3.0 ± 0.4 ml, respectively, and significantly different from those in the TF1 (20 μmol/l) group (n = 6, Fig. 4, P < 0.05 or P < 0.01), but not different from those in the control group. These data suggest that the cardioprotective effects of TF1 (20 μmol/l) can be abolished by atractyloside (20 μmol/l).

Discussion

In this study, the Langendorff technique was employed, and TF1 in three common concentrations of 10, 20 and 40 μmol/l [14] was used to investigate the effect of TF1 on isolated I/R heart for the first time. The results show that short-term administration of TF1 before ischemia has a clear protective effect against I/R injury on the heart in young rats, manifested as an improved recovery of post-ischemic ventricular function. The protective effects of TF1 could be abolished by glibenclamide, a K_ATP antagonist, 5-HD, a selective mitochondria K_ATP antagonist, and atractyloside, an mPTP opener, which suggests that K_ATP and mPTP are involved in the cardiac protection afforded by TF1.

ATP-sensitive potassium (K_ATP) channels exist in high density in the sarcolemmal membrane as well as the mitochondrial membrane of cardiomyocytes. The K_ATP channel is a weakly inward-rectifying K⁺ channel that is inhibited by intracellular ATP and activated by intracellular nucleoside diphosphates. Under physiological conditions, the K_ATP channel exists mainly in a closed, inactive form. The probability of the channel opening, however, is increased during myocardial ischemia, as the intracellular ATP concentration falls and ischemic metabolites (ADP, lactate, H⁺) accumulate. This results in an enhanced outward repolarizing flow of K⁺ and cell membrane hyperpolarization. Consequently, the myocardial action potential duration (APD) is shortened, and the voltage-dependent calcium current and myocardial contractility are decreased in which ATP is preserved during ischemia. Generally, it is thought that K_ATP channels have a protective property in myocardial ischemic diseases [15]. In this study, the cardioprotection of TF1 was abolished by glibenclamide, a non-selective K_ATP inhibitor, suggesting K_ATP channels are involved in the protective effect of TF1. In recent years, it was found that an ATP-sensitive K⁺ channel in the mitochondrial inner membrane was involved in the signaling cascade of myocardial ischemic preconditioning and that it played an important role in cardiac protection against myocardial ischemic injuries [16]. A number of studies have proved the role of mitochondrial K_ATP channels in ischemic and pharmacological preconditioning based on the ability of 5-HD to block cardioprotection [17, 18]. In our study, the addition of 5-HD, the specific mitochondrial K_ATP channel blocker, abolished mostly the cardioprotection of TF1 on reperfusion-induced injury, which suggests that the K_ATP channel, especially the mitochondrial K_ATP channel, may be involved in the cardioprotective effect of TF1. A recent study on theaflavins has demonstrated that a PKCε-dependent regulation is involved in myocardial contraction [19]. It was reported that PKC activation resulted in opening of the mitochondrial K_ATP channel and consequently induced the postconditioning of human myocardium [20]. Thus, we guess that the opening of the mitochondrial K_ATP channel in TF1 cardioprotection resulted from the activation of PKC.

Myocardium is a typically aerobic tissue, and its metabolism totally depends on oxygen availability in mitochondria. It was confirmed that I/R could damage the mitochondrial functions, including depression of energy production, disruption of ionic homeostasis and generation of free radicals [21]. The mPTP is a non-specific large pore in the inner mitochondrial membrane and usually opens in response to oxidative stress during reperfusion of the ischemic myocardium. The mPTP opening allows water and solutes to enter the mitochondria, leading to matrix swelling, inner membrane potential collapse, uncoupling of the respiratory chain, efflux of Ca²⁺ and release of small proteins such as cytochrome c [22]. Recent studies have found that suppression of mPTP opening during the first few minutes of reperfusion may be important for IPC [21, 23]. The inhibitors of mPTP opening, such as cyclosporin A (CsA) and sanglifehrin A, have already been shown to protect the heart against I/R injury [24–26]. In this study, atractyloside, an mPTP opener, completely abolished the protective effects of TF1, suggesting that reduction of mPTP opening during reperfusion plays an important role in the cardiac protection of TF1.

Ca²⁺ overloading induces mPTP opening, which appears to be a critical event in the transition from reversible to irreversible myocardial injury following an ischemic insult [21, 27]. This permeability transition leads to the collapse of the mitochondria membrane potential, massive mitochondrial swelling and loss of low-molecular weight components (such as cytochrome c) from the intermembrane place, which contributes to cell death [28, 29]. Opening of mitochondrial K_ATP channels dissipates mitochondrial membrane potential and releases Ca²⁺ from mitochondria into the cytoplasm, leading to a decrease in the driving force for Ca²⁺ uptake into the mitochondria and prevents mitochondrial Ca²⁺ overloading. A previous study showed that mitochondrial K_ATP channel activation might inhibit mPTP opening at reperfusion [21], but the mechanism is not clear, and the link between mitochondrial K_ATP channels and mPTP needs further investigation.

In summary, the present study demonstrated firstly that TF1 protects the rat heart against I/R injury through the opening of K_ATP channels, particularly on the mitochondrial membrane, and secondly inhibits mPTP opening.