Fig. 3

Evaluation for the proliferative potential of nail stem cell. a HE staining of the nail organ (top). TZ transitional zone. NM nail matrix zone. Immunostaining (brown) of pH3 (middle) and PCNA (bottom). Scale bars: 100 µm. b No significant difference in the ratio of TZ to NM among the groups (mean ± SE). Each point represents one individual. CA (N = 7), DT (N = 4), PA (N = 7). c The ratio of TZ to NM did not differ between DT and PA in the same individual (N = 4) (ns, paired t-test). d–g Quantification of the number of cells positive for pH3 or PCNA normalized to the NM area (mean ± SE). d–f Each point represents one individual. CA (N = 7), DT (N = 4), PA (N = 7) (Tukey–Kramer test). e–g The number of cells positive for pH3 or PCNA normalized to the NM area did not differ significantly between DT and PA in the same individual (N = 4) (ns, paired t-test)