Fig. 4
Re-expression of THADA in the THADA-knockdown cells. A, B The KB cells transfected the THADA siRNA (siTHADA) for 24 h were further treated with human THADA-expressing vector (hTHADA) or empty vector (mock), and the medium was changed 5 h after treatment. In A, the cells were subsequently cultured for 24 h, and the lysate samples were prepared. Western blotting of the samples (7.5 μg/lane) was performed with anti-THADA and anti-β-actin antibodies. As a control, the lysate sample (7.5 μg/lane) from KB cells treated with negative control siRNA (siNC) for 48 h was used. Typical images of the blotting were shown. In B, the siTHADA-transfected cells were seeded (2 × 104 cells; knockdown). Then, the cell numbers of hTHADA- or mock-transfected cells were counted 0 h and 24 h after the subsequent culture. n = 8. **P < 0.01 versus mock