Fig. 3
Inhibition of cancer cell proliferation by THADA-knockdown. A Expression of THADA protein (190 kDa) in KB cells treated with THADA siRNA (siTHADA) or negative control siRNA (siNC) for 48 h. Western blotting was performed by using the lysate samples (15 μg/lane) and anti-THADA antibody. Typical image of the blotting was shown. B Immunocytochemistry of THADA in KB cells treated with siTHADA or siNC or 48 h. Localization of THADA (green) and β-actin (red) was shown. DAPI was used for staining nucleus (blue). Scale bars, 10 μm. C KB cells were treated with siTHADA or siNC. The siTHADA- or siNC-transfected cells were seeded (2 × 104 cells; 0 h). Then, the cell number was couted 24, 48, and 72 h after the treatment. n = 9. **P < 0.01 versus siNC