Fig. 2

Inhibition of expression of THADA protein by cardiac glycosides. HepG2 cells (A) and KB cells (B) were treated with and without 30–1000 nM ouabain (left), oleandrin (middle) or digoxin (right) for 24 h. As a control, the cells were cultured in the absence of cardiac glycosides for 24 h (cont). Then, the lysate samples (15 μg/lane) were prepared, and Western blotting was performed. Typical images of the blotting with anti-THADA and anti-β-actin antibodies were shown in upper panels. Expression level of THADA (190 kDa) was normalized by corresponding β-actin expression (45 kDa), and the quantitative data were shown in lower panels. n = 4–6. *P < 0.05 and **P < 0.01 versus control (cont)