Fig. 4
Analysis of the initial wave and later oscillatory phase of caffeine-assisted oscillation, focusing on GABAB receptors. Field potential recording was performed, and electrical stimulation was delivered in the same manner shown in Fig. 3A. A1 Waveforms obtained by field potential recording. Stable oscillation was generated in the caffeine-containing medium (top). Application of 10 μM baclofen blocked the oscillatory phase, but not the initial phase (middle). After washout of baclofen, the oscillatory phase reappeared (bottom). A2 Averages of wavelet number before and during baclofen application to caffeine-containing medium, and after washout of baclofen. A3 Areal integrated values of the initial wave before and after application of baclofen. Representative waveforms before and during baclofen application are superimposed and shown in the graph. Note that application of baclofen clearly decreases initial wave size. B1 Averages of wavelet number before and during baclofen (1 μM) application to caffeine-containing medium, and after washout of baclofen. B2 Areal integrated values of initial wave before and after application of baclofen. Note that application of baclofen tends to decrease the initial wave size. B3 Averages of wavelet number before and during baclofen (0.1 μM) application to caffeine-containing medium, and after washout of baclofen. B4 Areal integrated values of initial wave before and after application of baclofen. Note that application of low-concentration baclofen does not seem to affect initial wave size. Asterisks (*P < 0.005; **P < 0.001) indicate significant statistical differences