Fig. 4
Western blot (WB) analysis of the ratios of P-PKA/PKA and P-CREB/CREB following 6-week 3-OBA and ET intervention. A, E WB analysis of P-PKA and PKA. B, F WB analysis of P-CREB and CREB. C, G P-PKA/PKA ratio. D, H Ratio of P-CREB/CREB. Three bands per group of rats are used. Relative levels were standardized to GAPDH or β-tubulin. Measurement data were presented as the mean ± SD. The significance of the difference of the two groups C and H was calculated with the independent samples t test, and the four groups (S, 3, SH, and 3H) were analyzed with the two-way ANOVA. *p < 0.05. The number of biological repeats (n = 10)