Fig. 10

Involvement of TRPV1 and ASICs in NGF- and GDNF-induced hypersensitivity. High NGF (0.8 μM, 20 μL, i.m.)-induced decrease in MMWT was reversed by capsazepine (50 μM, 20 μL) but not by amiloride (50 mM, 20 μL) (a), whereas high GDNF (0.03 μM)-induced decrease in MMWT was reversed by amiloride but not capsazepine (b), suggesting that TRPV1 is involved in high NGF-induced hypersensitivity, whereas ASICs are involved in high GDNF-induced hypersensitivity. A mixture of low NGF- and low-GDNF-induced decrease in MMWT was reversed by both capsazepine (c) and amiloride (d), suggesting that both receptor channels are involved in low mixture-induced hypersensitivity. Mean ± SD. a, c, and d: Modified from Murase et al. [19]; b: Modified from Murase et al. [38]