Fig. 3

Prediction of cellular urate transport direction from the expression of the transporters. A Models indicating all potential expression patterns of urate transporters. Rows indicate cell populations: AIP cell population, BIP cell population, and DIP cell population. Columns indicate expressions of efflux transporters; AE: Apical Efflux transporter; BE: Basolateral Efflux transporters. Colors show the types of transporters: red, AI or BE transporters; blue, BI or AE transporters. Arrows indicate urate transport directions. Functional cells for urate transport are marked with circles in which the circle colors indicate cellular transport directions: red, reabsorption; blue, secretion; and green, bi-directional. B Proportion of the cell populations in renal anatomical regions. The bar plot shows the percentage of cells (y-axis) in each cell population across the PT and LOH (DL) clusters (x-axis). C Dot plot indicates the frequency and expression levels of SLC2A9 (x-axis) across the cell populations (y-axis) along the three PT segments (S1–S3) and DL. Dot sizes refer to the frequency of a molecule expressed in the cell population (%Exp), while dot colors indicate expression levels (Avg Exp). D Schematic diagram of SLC2A9 expression in the BIP cell population with the Apical Efflux transporters (AE(+)). The apical membrane is to the left of the cells and the basolateral membrane is to the right of the cells. Red symbols represent the AI transporters. Blue symbols represent the BI and AE transporters. Arrows indicate directions of urate flow. E Positivity proportion of SLC2A9 in the BIP cell population with the Apical Efflux transporters. The bar plot shows the percentage of cells (y-axis) in each cell population across the PT and LOH (DL) clusters (x-axis)