Fig. 7

The number of c-Fos-positive neurons and c-Fos-positive orexin-neurons in LH of 1-day or 8-day RF-mice. A and B, Top. Representative sections show c-Fos positive neurons (A) and c-Fos positive orexin neurons (B) in the LH of AL mice and RF mice for 1-day food restriction (Left top) and 8 days of food restriction (Right top), respectively. The scale bar is 50 μm. The mean (± SEM) of c-Fos positive neurons (A, Bottom) and the ratio of c-Fos-orexin double-positive neurons to orexin neurons (B, Bottom) for ten subjects are shown on the y-axis. *P < 0.05; **P < 0.01; ***P < 0.001 difference between AL mice and RF mice according to unpaired t-test with Welch’s correction