Fig. 5

Increase in the number of c-Fos-positive neurons in IC of 1-day or 8 days RF-mice. The mean (± SEM) number of c-Fos positive neurons for ten subjects is shown on the y-axis in the AI (A), MI (B), and PI (C). *P < 0.05; **P < 0.01 difference between AL and RF mice according to unpaired t-test with Welch’s correction. Top panels in A, B, and C. Representative sections show c-Fos positive neurons in the AI, MI, and PI of AL and RF mice for 1-day food restriction (Left top) and 8 days of food restriction (Right top), respectively. The scale bar is 50 μm