Fig. 10

Daily locomotor activity, food intake and body weight of mice during1 or 8-days restricted feeding. A and B Increased food anticipation activity (locomotor activity during ZT2-ZT4) in response to the restricted feeding in 8 days (A, n = 10) or 1 day (B, n = 10 during ad libitum feeding, n = 5 during food restriction) RF mice. C A similar daily total locomotor activity was observed in the 8 days RF mice to that of the AL mice. D Daily food intake fluctuation and (E) changes in daily body weight of the 8 days RF mice. The mean (± SEM) of daily food intake (D) or daily body weight (E) for ten subjects is shown on the y-axis. The x-axis represents experimental days. *P < 0.05 (A); ****P < 0.0001 (A and D); **P < 0.01 (E) difference between AL mice and RF mice according to repeated measures two-way ANOVA with Bonferroni’s multiple comparison test. According to repeated measures two-way ANOVA, there was no significant difference in locomotor activity during ZT2-ZT4 (B) or ZT0-ZT24 (C) between AL mice and RF mice