Fig. 7

The effects between miR-574-5p and ROCK2 overexpression on LPS-caused cell injury. HPAEpiCs were treated with LPS, LPS+miR-NC, LPS+miR-574-5p, LPS+miR-574-5p+pcDNA or LPS+miR-574-5p+ROCK2, with untreated HPAEpiCs as a control, and ROCK2 expression was determined by Western blotting (A), cell viability by CCK-8 assay (B), cell proliferation by EdU assay (C and D), cell apoptosis by flow cytometry analysis (E), the protein expression of Bax and cleaved-caspase-3 by Western blotting analysis (F), the secretion of IL-6 and TNF-α by ELISA (G), MDA level by lipid peroxidation MDA assay (H), and SOD activity by SOD activity detection assay (I). *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001