Fig. 6

The effect of ROCK2 knockdown in LPS-induced HPAEpiC injury. HPAEpiCs were treated with LPS, LPS+si-con, or LPS+si-ROCK2, with untreated HPAEpiCs as a control, and ROCK2 expression was determined by Western blotting (A), cell viability by CCK-8 assay (B), cell proliferation by EdU assay (C), cell apoptosis by flow cytometry analysis (D), the protein expression of Bax and cleaved-caspase-3 by Western blotting analysis (E), the secretion of IL-6 and TNF-α by ELISA (F), MDA level by lipid peroxidation MDA assay (G), and SOD activity by SOD activity detection assay (H). **P < 0.01, ***P < 0.001 and ****P < 0.0001