Fig. 5

MiR-574-5p combines with ROCK2. A Schematic illustration showing miR-574-5p-binding sites in ROCK2 3’UTR. B, C The interplay between miR-574-5p and ROCK2 was confirmed by dual-luciferase reporter assay and RIP assay. D ROCK2 expression was detected by qRT-PCR in blood samples from healthy volunteers and blood samples from sepsis patients. E ROC curve analysis of the diagnostic value of ROCK2 in ALI. F MiR-574-5p expression was detected by Western blotting analysis in LPS-stimulated HPAEpiCs and untreated HPAEpiCs. G Spearman correlation analysis was carried out to determine the linear correlation between miR-574-5p and ROCK2 expression in the blood samples from sepsis patients. H The efficiency of miR-574-5p overexpression or knockdown was determined by qRT-PCR in LPS-treated HPAEpiCs. I The effects of miR-574-5p overexpression and knockdown on LPS-increased ROCK2 production were determined by Western blotting. **P < 0.01, ***P < 0.001 and ****P < 0.0001