Fig. 4

The effects between circ_0114428 knockdown and miR-574-5p silencing on LPS-induced HPAEpiC injury. HPAEpiCs were treated with LPS, LPS+si-NC, LPS+si-circ_0114428, LPS+si-circ_0114428+anti-miR-NC or LPS+si-circ_0114428+anti-miR-574-5p, with mock HPAEpiCs as a control, and miR-574-5p expression was determined by qRT-PCR (A), cell viability by CCK-8 assay (B), cell proliferation by EdU assay (C and D), cell apoptosis by flow cytometry analysis (E), the protein expression of Bax and cleaved-caspase-3 by Western blotting analysis (F), the secretion of IL-6 and TNF-α by ELISA (G), MDA level by lipid peroxidation MDA assay (H), and SOD activity by SOD activity detection assay (I). *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001