Fig. 3

Circ_0114428 combines with miR-574-5p. A Schematic illustration showing miR-574-5p-binding sites on circ_0114428 and the mutated sites of circ_0114428. B The efficiency of miR-574-5p overexpression was detected by qRT-PCR. C, D Dual-luciferase reporter and RIP assays were employed to demonstrate the interplay of circ_0114428 and miR-574-5p. E MiR-574-5p expression was detected by qRT-PCR in blood samples from healthy volunteers (N = 19) and blood samples from sepsis patients (N = 23). F ROC curve analysis of the diagnostic value of miR-574-5p in ALI. G MiR-574-5p expression was detected by qRT-PCR in LPS-stimulated HPAEpiCs and untreated HPAEpiCs. H Spearman correlation analysis was carried out to determine the linear correlation between miR-574-5p and circ_0114428. I The efficiency of circ_0114428 overexpression was detected by qRT-PCR in LPS-treated HPAEpiCs. J The impact of circ_0114428 knockdown or overexpression on LPS-mediated miR-574-5p expression was determined by qRT-PCR. **P < 0.01, ***P < 0.001 and ****P < 0.0001