Fig. 4

Western blot analysis of signaling mediators affected by cardiomyopathy in EDMD mice. A Immunoblot images of signaling mediators affected by cardiomyopathy are shown. B Graphs represent the ratio between the phosphorylated forms of mTOR, AKT, Smad3, and ERK1/2, normalized to the total level of each target protein. Total levels of these proteins were normalized to the level of β-actin (n = 4 for WT and Emd at 18 weeks of age, and n = 6 for H222P and EH mice at 18 and 30 weeks of age). *P < 0.05, **P < 0.01, and ***P < 0.001 versus WT mice at 18 weeks of age. ^###P < 0.001 versus H222P mice at 30 weeks of age