Fig. 3

Evaluation of myocardial fibrosis by immunohistochemistry and western blotting. A Representative images of a section of the cardiac muscle from WT and EDMD model mice. Periostin-positive fibrotic areas were prominent around cardiomyocytes in the left ventricles compared with those in the right ventricles of both H222P and EH mice at 30 weeks of age. Scale bar represents 100 µm. B Western blots were performed on four individual samples from WT and Emd mice at 18 weeks of age, and six individual samples from H222P and EH mice at 18 and 30 weeks of age. C Graphs represent the quantification of fibrotic markers PDGFRα, α-SMA, and β-catenin levels normalized to the level of β-actin. *P < 0.05, **P < 0.01, and ***P < 0.001 versus WT mice at 18 weeks of age