Fig. 6

BAG3 specifically directs HSP70 to form a complex for degradation through the autophagy pathway. A Immunofluorescence staining of chaperone HSP70 (green) and cochaperone BAG3 (red). Colocalization of HSP70 and BAG3 is demonstrated as a yellow area indicating the association of HSP70 and BAG3; white arrows denote the colocalization of HSP70 and BAG3. Scale bars = 20 μm. B Immunofluorescence staining of chaperone LC3 (green) and cochaperone BAG3 (red). Colocalization of LC3 and BAG3 is demonstrated as a yellow area indicating the association of LC3 and BAG3; white arrows denote the colocalization of LC3 and BAG3. Scale bars = 20 μm. C, D Analysis of the immunoreactive areas of BAG3 and HSP70. E Statistical analysis of HSP70 and BAG3 colocalization dots (n = 15). F Statistical analysis of LC3 and BAG3 colocalization dots (n = 15). ^#P < 0.05 vs. Group C, ^*P < 0.05 vs. Group EE