Fig. 5

Ubiquitinated substrates were recognized by p62 in muscle fibers. A Immunofluorescence staining of ubiquitin (green) and autophagic cargo p62 (red). Colocalization of ubiquitin and p62 is demonstrated using a yellow area indicating the association of ubiquitin and p62; white arrows denote the colocalization of ubiquitin and p62 dots. Scale bars = 20 μm. B Analysis of the immunoreactive area of ubiquitin. C Statistical analysis of ubiquitin and p62 colocalization dots (n = 15). ^#P < 0.05 vs. Group C, ^*P < 0.05 vs. Group EE