Fig. 3

Autophagic cargo p62 associated with the autophagosome marker LC3-1n muscle fibers. A Immunofluorescence staining of the autophagosome marker LC3 (green) and autophagic cargo p62 (red). Colocalization of LC3 and p62 is demonstrated with yellow dots indicating autolysosome association of LC3 and p62; white arrows denote the colocalization of LC3 and p62. Scale bars = 20 μm. B Analysis of the immunoreactive area of p62. C Statistical analysis of LC3 and p62 colocalization dots (n = 15). ^#P < 0.05 vs. Group C, ^*P < 0.05 vs. Group EE