Fig. 2

Assessment of the degradation of autophagosomes in gastrocnemius muscle tissue. A Immunofluorescence staining of autophagosome marker LC3 (green) and lysosome marker LAMP-2 (red). Colocalization of LC3 and LAMP-2 is demonstrated using yellow dots indicating autolysosomes; white arrows denote autolysosomes. Scale bars = 20 μm. B, C Analysis of immunoreactive areas of LC3 and LAMP-2. D Statistical analysis of LC3 and LAMP-2 colocalization dots (n = 15). ^#P < 0.05 vs. Group C, ^*P < 0.05 vs. Group EE