Fig. 1

Alterations of autophagosomes in gastrocnemius. A Representative blots images of autophagy-related proteins Beclin 1, p62, LC3, and GAPDH in gastrocnemius muscle tissue. B–F Qualitative analysis of autophagy-related proteins (n = 8); B protein levels of Beclin 1, C protein levels of p62, (D, E) protein levels of LC3-1 and LC3-II, and (F) the ratio of LC3-II/LC3-I. G TEM images of autophagic changes in muscle fibers; black arrows show autophagic changes. Scale bars = 0.5 μm. ^#P < 0.05 vs. Group C, ^*P < 0.05 vs. Group EE