Fig. 2

HE and toluidine-blue staining indicate that noradrenaline-storing cells are mast cells. A–G In the areas of each tissue where noradrenaline-storing cells are observed in immunofluorescent analyses shown in Fig. 1, cells with storage granules were stained with basophilic color (arrows). The morphology and localization of stained cells indicates that they were mast cells. Scale bars: 100 µm. H–N Toluidine blue staining was performed to confirm the presence of mast cells in the same areas. Scale bars: 100 µm