Fig. 2

Typical example of the effect of AM251 on the slow ventral root potential (sVRP) in newborn rat spinal cord preparations. A Time course of changes in the peak amplitude of the sVRP in response to the application of 10 µM AM251. B Examples of the reflex response in control (a), at washout immediately after the 15 min administration of AM251 (b), and after 15 min washout (c). a–c correspond to a-c in panel A. Note the gradual increase in the sVRP amplitude after treatment with AM251. C Data plots of the sVRP amplitude from 7 preparations at control (black), 15 min application of AM404 (red), and 15 min after washout (blue). Bars on the right side of the individual datasets denote the mean ± S.D. The average values of the sVRP amplitude were significantly increased. ** P < 0.01, *** P < 0.001, by a one-way repeated-measures ANOVA, followed by a Tukey‒Kramer multiple comparisons test