Fig. 4

Detection of progeny from each NSC. a Schematic diagram of the Ara-C infusion, followed by the neurosphere assay. b Schematic diagram of the clonal isolation of lentivirus-infected NSCs and inverse PCR to identify the lentivirus integration sites. c DNA from each neurosphere clone, the olfactory bulb, and the cortex, and mixed DNA from the other neurosphere clones (Other NSs) derived from the same brain were subjected to integration site-specific nested PCR. Representative PCR results from a DN-NSC (left, Clone #82-10-3), duplicate NSCs (middle, Clones #82-10-1 and #82-10-13), and an OB-NSC (right, Clone #82-10-12) from the same brain of an Ara-C-infused mouse are shown. d Analysis of the 53 and 69 independent GFP⁺ neural stem cell clones derived from PBS- (left panel) and Ara-C-infused (right panel) mice, respectively, is shown. e Schematic diagram of our model. DN-NSCs undergo symmetric division to produce two daughter NSCs, which can be detected as duplicate NSCs until one or both progeny differentiate into transit amplifying cells (TACs) or die. After one daughter cell is consumed to provide olfactory bulb neurons, the other NSC can be detected as an OB-NSC until it is ultimately lost