Fig. 3

Tamoxifen injection and Ara-C infusion experiments. a Schematic diagram of the Ara-C infusion experiment following tamoxifen injection. b Tamoxifen induces recombination between two LoxP sequences within the lentiviral genome in Nestin-lineage cells. PCR primers to detect the recombination are shown. c Primary neurospheres including GFP⁺ neurospheres are shown. d DNA from amplified daughter neurospheres clonally derived from individual GFP⁺ spheres was subjected to recombination-detecting PCR. Unfloxed and floxed controls indicate PCR amplification from the lentiviral genome before and after recombination, respectively. e Schematic diagram of the Ara-C infusion experiment following tamoxifen injection using Nestin-CreER::Ai14 mice. Most of primary neurospheres generated 4 weeks after the Ara-C treatment expressed tdTomato. Arrowheads indicate a tdTomato^– neurosphere. Scale bars, 100 µm