Fig. 1

Lentivirus infection of neonatal mouse brains. a Structure of GFP-expressing lentivirus with two LoxP sequences and primers are shown. b Schematic diagram of lentiviral infection in neonatal mouse brains and analysis of adult brains. c Numbers of primary neurospheres generated from 2500 cells in the SEZ of the right hemisphere are shown. d Total numbers of neurosphere-forming NSCs in the right hemisphere over the lifespan are determined and shown along with an exponential graph (dotted line) that was calculated from the data between 10–54 weeks (open circles). A number of NSCs in a mouse at 104 weeks was overlaid on the graph (a closed circle). Data represent the mean ± SEM. *: P < 0.05 by one-way ANOVA followed by Tukey’s post hoc comparison