Fig. 5

Antagonism of GABA B-Rs in the GP inhibits the activity of RTn neurons and the reduction in the power spectra in the cortical beta band. A Histogram of one RTn neuron firing activity after GP administration of saclofen (300 ng). The red line represents the injection period. Right: Spiking rate of RTn neurons under basal conditions and after pharmacological stimulation with 300 ng of saclofen. The firing rate of RTn cells decreased relative to the basal values after local injection of saclofen; p = 0.000001, paired t test; n = 12 neurons). The same doses of saclofen increased the spiking activity in four neurons (basal = 7.12 ± 0.94 spikes/s, saclofen = 10.75 ± 1.37 spike/s; p = 0.005, paired t test, n = 4 neurons). B Spectrograms of the RTn and cortical activity under experimental conditions (5-s bin). The blockade of GABA B-Rs by 300 ng of saclofen in the GP did not modify the spectral density of the cortical beta band after reducing the firing rate of RTn neurons. Top: Raster plot representing the RTn neuron activity in the basal state and after pharmacological blockade. Bottom: Traces illustrating the cortical activity under both basal and baclofen conditions. The time scales apply to both traces. The plot was derived from the same neuron. C Left: The plot shows the average power spectral density under the experimental conditions. The blockade of GABA B-Rs in the GP with 300 ng of saclofen does not modify the spectral density in the 13–19Hz frequency. However, an increase in the power spectral density is observed in the frequency range of 20–30 Hz. Right: Statistics on the effect of the application of saclofen to the ipsilateral GP at 13–19 Hz (p = 1, paired t test; n = 12 neurons, top) and 20–30 Hz (p = 0.00365, paired t test; n = 12 neurons, bottom). D Left: Graph showing a lack of coherent activity at frequencies associated with the beta band between the MCx and RTn neuron activity. The dashed lines represent the 95% confidence intervals. Right: The graph represents the percent response of the RTn neurons after antagonism of GABA B-Rs in the GP