Fig. 6

Effect of knockdown of RhoA, RhoB and RhoC on thrombin-induced di-phosphorylation of myosin light chain (ppMMLC) in porcine aortic endothelial cells (PAECs). Representative immunoblot images and summaries of immunoblot detection of RhoA, RhoB and RhoC (a), and ppMLC before and 3 min after stimulation with 1 unit/mL thrombin (b). The samples obtained before thrombin stimulation were used to detect Rho proteins. The data are expressed as mean ± SEM (n = 4). *P < 0.05 vs. the other conditions according to an ANOVA followed by Fisher post hoc test. In b, the values of siRhoA, siRhoB and siRhoC before and after thrombin stimulation showed no significant difference from those of siControl, according to an ANOVA followed by Dunnett’s post hoc text. All original images of the immunoblot analyses are shown in Additional file 1: Figure S6