Fig. 1

Effects of simvastatin pretreatment on the thrombin-induced decrease in trans-endothelial electric resistance (TEER) and mono- and di-phosphorylation of myosin light chain (pMLC, ppMLC) in porcine aortic endothelial cells (PAECs). a, b The time courses of thrombin-induced changes in TEER (a, n = 6), pMLC and ppMLC (b, n = 4) are summarized. c A representative immunoblot and the summary for the concentration-dependent effects of simvastatin on pMLC and ppMLC at 3 min after thrombin stimulation are shown (n = 5). PAECs at confluence were pretreated or left untreated with simvastatin at the indicated concentrations for 16 h. The cells were then stimulated with 1 unit/mL thrombin, in the presence of simvastatin, when pretreated. All original images of the immunoblot analyses are shown in Additional file 1: Figure S1. The data are expressed as the mean ± SEM. *P < 0.05 vs. thrombin, according to an ANOVA followed by Dunnett’s post hoc test