Fig. 5

Representative example of the averaged fluorescence signal triggered by C4 inspiratory activity. Aa–f From top to bottom, each trace shows the average fluorescence signal obtained at each depth from the focus (0, 10, 20, 30, 40 and 50 μm), respectively, triggered by the C4 inspiratory activity in the strong (R1 and R2), weak (R3 and R4), and no (R5 and R6) inspiratory modulation groups. Ag From upper to lower, the averaged wave of leaky integrated C4 activity obtained at each depth from the focus (arbitrary unit). B Summary graph showing the amplitude of the inspiratory peak at each depth of focus. The data from the strong (n = 15), open circles; weak (n = 10), open triangles; and no (n = 13), filled circles inspiratory-modulated groups are shown. Note that the open circles gradually decrease with increasing depth, whereas the other symbols tend to be constant and at approx. 1% at any depth