Fig. 6

Analysis of myocardial apoptosis on day 8 post-RMI. a Representative micrographs of TUNEL, Cox43 (connexin 43), and DAPI staining of the I/R region. Scale bars, 50 μm. b Quantitative analysis of apoptotic cells (UT: 213 ± 3/field, n = 4, 24 fields; DT: 99 ± 8/field, n = 6, 36 fields; MT: 236 ± 31/field, n = 5, 30 fields; DMT: 39 ± 2/field, n = 4, 24 fields). Data are expressed as means ± SEMs. B Box-and-whisker plots based on apoptotic cells in all fields of each group. **P < 0.01 vs. UT; ^††P < 0.01 vs. MT, using nonparametric Kruskal–Wallis tests, followed by Dunn’s test