Fig. 4

Neutrophil infiltration of rat myocardium and histological analysis on day 8 after reperfused myocardial infarction (RMI). Representative photomicrographs of Masson’s trichrome staining (a), and hematoxylin and eosin staining of the heart (b) and the ischemia/reperfusion (I/R) region. The whole heart scale bars are 2 mm. Infiltrating neutrophils in the I/R region, scale bars are 50 μm. c Box-and-whisker plots of neutrophil counts in the I/R region (UT: 271 ± 15/field, n = 5, 30 fields; DT: 123 ± 7/field, n = 5, 30 fields; MT: 257 ± 10/field, n = 4, 24 fields; DMT: 136 ± 4/field, n = 4, 24 fields). Data are expressed as means ± SEMs. **P < 0.01 vs. UT; ^††P < 0.01 vs. MT, using nonparametric Kruskal–Wallis tests, followed by Dunn’s test