Fig. 2

Total and ubiquitinated protein expression on days 1 and 7 following hindlimb unloading. CON, control; LOS, losartan. Ubiquitinated protein expression in the cytosol (a and b) and myofibril (c and d) before (PRE), and on days 1 and 7 following hindlimb unloading, and representative blots. Values are represented as mean ± standard deviation; n = 7 per time point. Normally distributed data were analyzed using two-way ANOVA. For non-normal distribution, the Kruskal–Wallis test was performed, followed by the two-stage step-up method of Benjamini, Krieger, and Yekutieli; the test was used for the analysis within each sex. *P < 0.05 vs. PRE of each sex