Fig. 7
From: Bicarbonate transport of airway surface epithelia in luminally perfused mice bronchioles
Messenger RNA expression of ion transporters and channels in bronchiole epithelial cells. Messenger RNA was extracted from tracheal mucosa (T), isolated bronchioles (B), colon (C), kidney (K), stomach (S), heart (H), lung (L), and brain (Br) and reverse transcribed. PCR was performed using each cDNA as template and with gene-specific primers (Table 1). GAPDH was used as a reference. ‒: PCR was performed in the absence of RT enzyme. M: 100-bp DNA ladder