Fig. 2

MiR-18a-3p targets PDP1. A Potential target genes (SNX8, FNDC5, EFNA1, ZBBX, ADCY1, PDP1, UBE2Z and THSD7B) of miR-18a-3p were predicted by miRDB. B The expression of potential target genes in ATDC5 cells transfected with miR-18a-3p mimics or miR-NC was detected by RT-qPCR. C The binding site of miR-18a-3p for PDP1 3′UTR was predicted by TargetScan. D Luciferase reporter assay was used to validate the binding relation between miR-18a-3p and PDP1 3′UTR in ATDC5 cells. E, F PDP1 protein level in ATDC5 cells after overexpressing miR-18a-3p was examined by western blotting. **p  < 0.01