Fig. 1
(with permission of ref. [13])
A Representative flow cytometry recordings of RAR-related orphan receptor-γ (RORγt)-positive cells (blue) in the lamina propria of upper and lower parts of jejunum and ileum in the control. The ordinate and abscissa of each panel show fluorescent intensity of CD3-FITC and RORγt-PE, respectively. For flow cytometry analysis, the gating strategy to isolate ILC3s from the collected cells was as follows: (1) isolation of the live cells, (2) gating and isolation of CD-45 positive cells, and then (3) gating and isolation of CD-45R- and CD-3-negative and RORγt-positive cells. B Effects of 2-h water intake on expression of IL-22 mRNA in the lamina propria of upper and lower parts of jejunum and ileum. Expression levels of IL-22 mRNA were normalized relative to those of β-actin (ordinate, n = 5). Open and dappled bars show control and treatment with 2-h water intake, respectively. **p < 0.01; NS not significant. C Effects of 2-h water intake on composition (top) and total number (bottom) of innate lymphoid cells 3 (ILC-3; n = 5) in upper and lower parts of the jejunum and in mesenteric lymph node (the ordinate). Open and dappled bars show control and treatment with 2-h water intake, respectively. *p < 0.05