Fig. 3

MiR-200c-3p inhibited SMAD7 and YAP expression. A Luciferase reporter assay was performed to verify the relationship between miR-200c-3p and 3′ UTR of SMAD7. The qRT-PCR (B) and WB (C) were performed to assess the expression of SMAD7 in the HUVECs following transfection of miR-200c-3p mimic, mimic NC, miR-200c-3p inhibitor, or inhibitor NC. D Co-IP assay was performed to determine the interaction between SMAD7 and YAP in HUVECs. The qRT-PCR (E) and WB (F) were performed to assess the expression of YAP in the HUVECs following transfection of miR-200c-3p mimic, mimic NC, miR-200c-3p inhibitor, or inhibitor NC. **P < 0.01 vs. mimic NC group; ^##P < 0.01 vs. inhibitor NC group