Fig. 3

Functional mapping of Fos expression in nesfatin-1/NucB2-ir neurons after chemogenetic activation of endogenous AVP. A Digital images of fluorescent immunohistochemistry for nesfatin-1/NucB2 (red) and Fos (green) in the lateral hypothalamic area (LHA), central nucleus of the amygdala (CeA), locus coeruleus (LC), rostral part of nucleus tructus solitalis (rNTS), caudal part of nucleus tructus solitalis (cNTS), and rostral ventrolateral medulla (RVLM). Each schematic illustration indicates nesfatin-1/NucB2-ir neurons (white circle) and co-localization of nesfatin-1/NcB2 neurons expressing Fos (red circle) in each nucleus. The images were obtained from the rats at 120 min after s.c. injection of Saline or CNO. The scale bars indicate 200 µm. Fx, fornix; 4V, fourth ventricle. B The percentages of nesfatin-1/NucB2-ir neurons expressing Fos-ir at 120 min after s.c. injection of CNO. Data are presented as means ± SEM (n = 5, each). *P < 0.05, **P < 0.01 vs. Saline-treated group. VTA ventral tegmental area, DR dorsal raphe nucleus, AP area postrema. DR were divided into four following subregions; dorsal (DRd), ventral (DRv), ventrolateral “wings” (DRvl), and inter-fascicular (DRi)