Fig. 4
Attenuation of cardiac NO synthesis potential and vagus nerve activity with altered ryanodine receptor expression in the ChATKD hearts. a A NO metabolite, NO2, in the hearts is significantly lower in ChATKD hearts than in WT hearts (P < 0.0001) due to depressed NOS1 (P = 0.048) but not NOS3 expressions in the hearts. b Neuronal activity of the vagus nerve is lower in ChATKD mice than in control mice (P = 0.019). c Immunoreactivity of ryanodine receptor (RYR) is exclusively more enhanced in cytoplasm of the cardiomyocytes in ChATKD hearts than in control hearts. Scale bar: 10 μm. d RYR, whose protein expression is enhanced in ChATKD hearts, and NOS1 interaction (arrow) is impaired in the ChATKD hearts