Fig. 2
Two-photon image of the left parietal cortex in APPNL-G-F mice. Examples of three-dimensional imaging obtained from two individual mice are presented (a, b). Blood plasma and Aβ are labeled using fluorescent dyes. An image enclosed in a white frame in each panel is a stack image with a maximal intensity projection, and the remaining images are a series of horizontal images. In a, a stack image constructed with z-stacks of 320 μm and slice images are presented every 24 μm. In b, a stack image constructed with z-stacks of 200 μm and slice images are presented every 12 μm. Deposited Aβ in the cerebral parenchyma is enclosed by a dashed circle, and Aβ deposited around the vasculature is indicated by arrow heads. Scale bar indicates 50 μm