Fig. 7

Effects of reagents decreasing cellular ATP levels on the subcellular localization of GLUT1. a HUVECs were treated with antimycin-A (Anti-A, 10 μM) or 2-deoxyglucose (2-DG, 1 mM) with or without glibenclamide (Glb, 10 μM) under normoxia. The cellular distribution of GLUT1 was determined by immunofluorescence staining as described in the Materials and Methods. HUVECs were stained with an anti-GLUT1 antibody (green). Images were obtained with a confocal fluorescence microscope as described in the “Materials and methods”. Representative images are shown from 4 independent experiments with similar results. Scale bars, 25 μm. b The number of cells in which GLUT1 was observed at the cell membrane was counted in approximately 100 cells from 4 or 5 independent experiments. Data are the mean ± SEM from 4 independent experiments. *P < 0.01 versus normoxia control without glibenclamide treatment (unpaired t-test). ^#P < 0.01 versus antimycin-A without glibenclamide treatment (unpaired t-test). ^$P < 0.01 versus 2-deoxyglucose without glibenclamide treatment (unpaired t-test)