Fig. 6

Effects of reagents decreasing cellular ATP levels on the cell surface expression of GLUT1. HUVECs were treated with antimycin-A (Anti-A, 10 μM) (a) or 2-deoxyglucose (2-DG, 1 mM) (b) for 30 min at normoxia. In some groups, the cells were also treated with glibenclamide (Glb, 10 μM). The cells were labeled with a human anti-GLUT1 Alexa Fluor 700-conjugated antibody to detect GLUT1 on the cell surface. (a, b) The histograms represent cell counts (Y-axis, linear scale) versus fluorescence intensity (X-axis, log scale). Fluorescence mean intensity obtained from the histograms was quantified and shown in bar graphs (right panels). Data are the mean ± SEM from 4 independent experiments. *P < 0.01 versus normoxia control (Mann–Whitney test). ^#P < 0.01 versus antimycin-A (Mann–Whitney test). ^$P < 0.01 versus 2-deoxyglucose (Mann–Whitney test)