Fig. 2

Effect of hypoxia or reagents on the cell surface expression of GLUT1. a HUVECs were cultured under normoxia or hypoxia as described in the Materials and Methods. In some experiments, the cells were treated with glibenclamide (Glb, 10 μM). The cells were labeled a human anti-GLUT1 Alexa Fluor 700-conjugated antibody to detect cell surface GLUT1. b Effect of CoCl₂ and/or glibenclamide on the cell surface expression of GLUT1. HUVECs were treated with CoCl₂ (100 µM) and/or glibenclamide (Glb, 10 μM). GLUT1 at the cell surface was determined as described in (a). (a, b) The histograms represent cell counts (Y-axis, linear scale) versus fluorescence intensity (X-axis, log scale). Fluorescence mean intensity obtained from the histograms was quantified and shown in bar graphs (right panels). Data are the mean ± SEM from 4 independent experiments. *P < 0.01 versus normoxia control (Mann–Whitney test). ^#P < 0.01 versus hypoxia without glibenclamide treatment (Mann–Whitney test)